Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China; Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang, Guizhou 550005, China.
Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China; Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang, Guangxi 547100, China.
Nutr Res. 2014 Sep;34(9):780-8. doi: 10.1016/j.nutres.2014.08.008. Epub 2014 Aug 28.
The improvement of gut health and function with prebiotic supplements after weaning is an active area of research in pig nutrition. The present study was conducted to test the working hypothesis that medium-term dietary supplementation with soybean oligosaccharides (SBOS) can affect the gut ecosystem in terms of microbiota composition, luminal bacterial short-chain fatty acid and ammonia concentrations, and intestinal expression of genes related to intestinal immunity and barrier function. Ten Huanjiang mini-piglets, weaned at 21 days of age, were randomly assigned to 2 groups. Each group received a standard diet containing either dietary supplementation with 0.5% corn starch (control group) or 0.5% SBOS (experimental group). The results showed that dietary supplementation with SBOS increased the diversity of intestinal microflora and elevated (P < .05) the numbers of some presumably beneficial intestinal bacteria (e.g., Bifidobacterium sp, Faecalibacterium prausnitzii, Fusobacterium prausnitzii, and Roseburia). Soybean oligosaccharide supplementation also increased the concentration of short-chain fatty acid in the intestinal lumen, and it reduced (P < .05) the numbers of bacteria with pathogenic potential (e.g., Escherichia coli, Clostridium, and Streptococcus) and the concentration of several protein-derived catabolites (e.g., isobutyrate, isovalerate, and ammonia). In addition, SBOS supplementation increased (P < .05) expression of zonula occludens 1 messenger RNA, and it decreased (P < .05) expression of tumor necrosis factor α, interleukin 1β, and interleukin 8 messenger RNA in the ileum and colon. These findings suggest that SBOS supplementation modifies the intestinal ecosystem in weaned Huanjiang mini-piglets and has potentially beneficial effects on the gut.
在断奶后用益生元补充剂改善肠道健康和功能是猪营养的一个活跃研究领域。本研究旨在检验以下工作假设，即中期饮食补充大豆低聚糖（SBOS）可以影响肠道生态系统的微生物组成、腔内容物中细菌短链脂肪酸和氨浓度，以及与肠道免疫和屏障功能相关的基因的肠道表达。21 日龄断奶的 10 头环江小型猪被随机分配到 2 组。每组接受含有 0.5%玉米淀粉（对照组）或 0.5% SBOS（实验组）的标准饮食。结果表明，SBOS 饮食补充增加了肠道微生物群的多样性，并提高（P <.05）了一些假定有益肠道细菌（如双歧杆菌、普拉梭菌、普雷沃氏菌和罗氏菌）的数量。大豆低聚糖补充还增加了肠道腔短链脂肪酸的浓度，并降低（P <.05）了具有潜在致病性的细菌（如大肠杆菌、梭菌和链球菌）的数量和几种蛋白质衍生代谢物（如异丁酸、异戊酸和氨）的浓度。此外，SBOS 补充增加了 zonula occludens 1 信使 RNA 的表达（P <.05），并降低了回肠和结肠中肿瘤坏死因子 α、白细胞介素 1β 和白细胞介素 8 信使 RNA 的表达（P <.05）。这些发现表明，SBOS 补充改变了断奶环江小型猪的肠道生态系统，并对肠道具有潜在的有益作用。
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