Wei H-K, Xue H-X, Zhou Z X, Peng J
1Department of Animal Nutrition and Feed Science,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,People's Republic of China.
Animal. 2017 Feb;11(2):193-201. doi: 10.1017/S1751731116001397. Epub 2016 Jul 15.
Recent studies indicate that intestinal oxidative stress and microbiota imbalance is involved in weaning-induced intestinal dysfunction in piglets. We have investigated the effect of feeding a carvacrol-thymol blend supplemented diet on intestinal redox status, selected microbial populations and the intestinal barrier in weaning piglets. The piglets (weaned at 21 days of age) were randomly allocated to two groups with six pens per treatment and 10 piglets per pen. At weaning day (21 days of age), six piglets were sacrificed before weaning to serve as the preweaning group. The weaned group was fed with a basal diet, while the weaned-CB group was fed with the basal diet supplemented with 100 mg/kg carvacrol-thymol (1 : 1) blend for 14 days. On day 7 post-weaning, six piglets from each group were sacrificed to determine intestinal redox status, selected microbial populations, messenger RNA (mRNA) transcript levels of proinflammatory cytokines and biomarkers of intestinal barrier function. Weaning resulted in intestinal oxidative stress, indicated by the increased concentration of reactive oxygen species and thiobarbituric acid-reactive substances present in the intestine. Weaning also reduced the population of Lactobacillus genus and increased the populations of Enterococcus genus and Escherichia coli in the jejunum, and increased mRNA levels of tumor necrosis factor α (TNF-α), interleukin 1β and interleukin 6 (IL-6). In addition, decreased mRNA levels of zonula occludens and occludin in the jejunal mucosa and increased plasma diamine oxidase concentrations indicated that weaning induced dysfunction of the intestinal barrier. On day 7 post-weaning, supplementation with the carvacrol-thymol blend restored weaning-induced intestinal oxidative stress. Compared with the weaned group, the weaned-CB group had an increased population of Lactobacillus genus but reduced populations of Enterococcus genus and E. coli in the jejunum and decreased mRNA levels of TNF-α. The results indicated that weaning induced intestinal oxidative stress and dysfunction of the intestinal barrier. Dietary supplementation with 100 mg/kg carvacrol-thymol (1 : 1) decreased the intestinal oxidative stress and influenced selected microbial populations without changing the biomarkers of intestinal barrier in weaning piglets.
近期研究表明，肠道氧化应激和微生物群失衡与仔猪断奶诱导的肠道功能障碍有关。我们研究了在断奶仔猪日粮中添加香芹酚 - 百里香酚混合物对肠道氧化还原状态、特定微生物种群及肠道屏障的影响。仔猪（21日龄断奶）被随机分为两组，每组6个栏，每栏10头仔猪。在断奶日（21日龄），6头仔猪在断奶前宰杀作为断奶前组。断奶组饲喂基础日粮，而断奶 - CB组饲喂添加100 mg/kg香芹酚 - 百里香酚（1:1）混合物的基础日粮，持续14天。断奶后第7天，每组宰杀6头仔猪，以测定肠道氧化还原状态、特定微生物种群、促炎细胞因子的信使核糖核酸（mRNA）转录水平及肠道屏障功能生物标志物。断奶导致肠道氧化应激，表现为肠道中活性氧和硫代巴比妥酸反应性物质浓度增加。断奶还使空肠中乳酸杆菌属数量减少，肠球菌属和大肠杆菌数量增加，并使肿瘤坏死因子α（TNF-α）、白细胞介素1β和白细胞介素6（IL-6）的mRNA水平升高。此外，空肠黏膜中闭合蛋白和封闭蛋白的mRNA水平降低以及血浆中二胺氧化酶浓度升高表明断奶诱导了肠道屏障功能障碍。断奶后第7天，添加香芹酚 - 百里香酚混合物可恢复断奶诱导的肠道氧化应激。与断奶组相比，断奶 - CB组空肠中乳酸杆菌属数量增加，但肠球菌属和大肠杆菌数量减少，且TNF-α的mRNA水平降低。结果表明，断奶诱导了肠道氧化应激和肠道屏障功能障碍。在断奶仔猪日粮中添加100 mg/kg香芹酚 - 百里香酚（1:1）可降低肠道氧化应激并影响特定微生物种群，而不改变肠道屏障生物标志物。
