Park Hyoung Keun, Paick Sung Hyun, Kim Hyeong Gon, Lho Yong Soo, Bae Sang Rak
Department of Urology, School of Medicine, Konkuk University, Seoul, Korea.
Department of Urology, The Catholic University of Korea, Uijeongbu St. Mary's Hospital, Uijeongbu, Korea.
World J Mens Health. 2014 Aug;32(2):83-6. doi: 10.5534/wjmh.2014.32.2.83. Epub 2014 Aug 26.
The objective of the present study was to evaluate the efficacy of a sclerosing solution for inducing epididymal occlusion in male rats.
Male Sprague-Dawley rats were divided into two groups: an injection group (n=20) and control group (n=20). Before injecting the sclerosing agent, seminal vesiculectomy and sperm identification using electrostimulation were performed in all of the rats. In the injection group, 0.2 mL of 0.1% sodium tetradecyl sulfate solution was injected into the epididymis. In the sham group, only the identification of the epididymis was performed. At 4 and 12 weeks after the injection, semen was collected by electrostimulation and evaluated to assess the contraceptive effect. Epididymis was evaluated by hematoxylin and eosin (H&E) staining.
After 4 and 12 weeks, semen collection was performed in the two groups. Sperms were not observed in the injection group, while there was no change in the sperms in the sham group. H&E staining showed the obstruction of epididymal tubules and an accumulation of inflammatory cells in the injection group.
This study showed that the sclerosing agent induced sterilization in male rats. This result suggests that the injection method can replace vasectomy as a contraceptive method. However, a further study of large animals and a clinical study are needed. Further, the long-term effectiveness of this method needs to be studied.
本研究的目的是评估一种硬化剂溶液诱导雄性大鼠附睾闭塞的疗效。
将雄性Sprague-Dawley大鼠分为两组：注射组（n = 20）和对照组（n = 20）。在注射硬化剂之前，对所有大鼠进行精囊切除术并使用电刺激进行精子鉴定。在注射组中，将0.2 mL 0.1%的十四烷基硫酸钠溶液注入附睾。在假手术组中，仅对附睾进行鉴定。注射后4周和12周，通过电刺激收集精液并进行评估以评估避孕效果。通过苏木精和伊红（H&E）染色对附睾进行评估。
4周和12周后，对两组进行精液收集。注射组未观察到精子，而假手术组精子无变化。H&E染色显示注射组附睾小管阻塞和炎性细胞聚集。
本研究表明硬化剂可诱导雄性大鼠绝育。这一结果表明注射方法可作为一种避孕方法替代输精管切除术。然而，需要进一步对大型动物进行研究和开展临床研究。此外，还需要研究该方法的长期有效性。
