Sumayo Marilyn S, Kwon Duck-Kee, Ghim Sa-Youl
School of Life Sciences, KNU Creative Bioresearch Group (BK21 Plus Project) and Institute for Microorganisms, Kyungpook National University, Daegu 702-701, Republic of Korea.
Department of Biology Education, Kyungpook National University, Daegu 702-701, Republic of Korea.
J Plant Physiol. 2014 Nov 15;171(18):1757-62. doi: 10.1016/j.jplph.2014.08.015. Epub 2014 Sep 6.
Linoleic acid (LA) is a naturally occurring fatty acid (FA) found to elicit induced systemic resistance (ISR) of tobacco against the bacterial soft rot pathogen, Pectobacterium carotovorum subsp. carotovorum (PCC). In this study, we examined effects of six doses of exogenous LA on the induction of defense genes and enzymes. The optimum ISR activity was observed in plants treated with 0.1mM LA where the effect of LA on membrane permeability was minimal. The application of LA as a root drench enhanced the activity of defense enzymes such as phenylalanine ammonia-lyase (PAL), peroxidase (POD), and polyphenol oxidase (PPO) and induced the expression of β-glucuronidase (GUS). PAL and POD activities were increased in a concentration dependent manner while the maximum PPO activity was observed after treatment with 0.01mM LA. An RT-PCR analysis of the defense-related genes, Coi1, NPR1, PR-1a and PR-1b, of tobacco plants treated with 0.1mM LA revealed an association of LA with elicitation of ISR in tobacco.
亚油酸（LA）是一种天然存在的脂肪酸（FA），已发现它能引发烟草对细菌性软腐病原菌胡萝卜软腐果胶杆菌胡萝卜亚种（PCC）的诱导系统抗性（ISR）。在本研究中，我们检测了六种剂量的外源LA对防御基因和酶诱导的影响。在用0.1mM LA处理的植物中观察到最佳的ISR活性，此时LA对膜通透性的影响最小。以根灌方式施用LA增强了防御酶如苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和多酚氧化酶（PPO）的活性，并诱导了β-葡萄糖醛酸酶（GUS）的表达。PAL和POD活性以浓度依赖的方式增加，而在用0.01mM LA处理后观察到最大的PPO活性。对用0.1mM LA处理的烟草植株的防御相关基因Coi1、NPR1、PR-1a和PR-1b进行的RT-PCR分析揭示了LA与烟草中ISR诱导之间的关联。
