BMC Vet Res. 2014 Sep 20;10:219. doi: 10.1186/s12917-014-0219-2.
The snatch-farrowed porcine-colostrum-deprived (SF-pCD) pig model, in which neonates are raised on commercially available bovine colostrum, is an alternative model for porcine infectious disease research. It is not known if SF-pCD pigs possess growth performance and immunity comparable to conventional, farm-raised pigs. The current experiment compared growth performance and immune responses of SF-pCD pigs to their farm-raised siblings following Mycoplasma hyopneumoniae (Mhyo) vaccination. Twelve SF-pCD and 13 farm-raised siblings were vaccinated on day 7 (D7) and D26 of age. Body weights were measured once or twice weekly and average daily gain (ADG) was calculated. Peripheral blood mononuclear cells (PBMC) were isolated on D40. Cytokine secretion from PBMC stimulated with Mhyo antigen or phorbol myristate acetate plus ionomycin (PMA/Iono) was assessed using a multiplexed fluorescent microsphere immunoassay (FMIA). Additionally, interferon gamma (IFNγ) secretion from stimulated PBMC was assessed using ELISPOT. Mhyo IgG titers were measured by an ELISA in D40 sera.
Growth performance did not differ between groups before weaning, but SF-pCD pigs had higher ADG after weaning. In response to Mhyo stimulation, numbers of IFNγ secreting PBMC and levels of interleukin 8 (IL8) and IL10 in PBMC supernatants were significantly higher in SF-pCD pigs, as were Mhyo antibody levels in sera, and levels of IL1β, IL8 and IL12 in supernatants of PMA/Iono stimulated PBMC.
Under the conditions of this experiment, SF-pCD pigs demonstrated superior growth performance and enhanced humoral and cell-mediated immunity following vaccination. Whether or not this reflects greater resistance or tolerance to infection is unknown but the ability to react positively to the vaccination provides evidence that SF-pCD pigs are a suitable alternative model for swine disease research.
snatch-farrowed porcine-colostrum-deprived (SF-pCD) 猪模型，其中新生仔猪用市售牛初乳饲养，是一种替代猪传染性疾病研究的模型。目前尚不清楚 SF-pCD 猪是否具有与常规农场饲养猪相当的生长性能和免疫力。本实验比较了 Mhyo 疫苗接种后 SF-pCD 猪与其农场饲养兄弟姐妹的生长性能和免疫反应。12 只 SF-pCD 猪和 13 只农场饲养的猪在 7 日龄（D7）和 26 日龄（D26）接种疫苗。每周测量一次或两次体重，计算平均日增重（ADG）。在 D40 时分离外周血单核细胞（PBMC）。使用多重荧光微球免疫分析（FMIA）评估 PBMC 对 Mhyo 抗原或佛波醇肉豆蔻酸乙酸酯加离子霉素（PMA/Iono）刺激后的细胞因子分泌情况。此外，使用 ELISPOT 评估刺激后的 PBMC 中干扰素γ（IFNγ）的分泌。在 D40 血清中通过 ELISA 测量 Mhyo IgG 滴度。
断奶前两组的生长性能没有差异，但 SF-pCD 猪断奶后 ADG 更高。在 Mhyo 刺激下，SF-pCD 猪的 IFNγ 分泌 PBMC 数量以及 PBMC 上清液中白细胞介素 8（IL8）和白细胞介素 10（IL10）的水平显著升高，血清中 Mhyo 抗体水平以及 PMA/Iono 刺激 PBMC 上清液中白细胞介素 1β（IL1β）、白细胞介素 8（IL8）和白细胞介素 12（IL12）的水平也显著升高。
在本实验条件下，SF-pCD 猪表现出更好的生长性能和增强的体液和细胞介导免疫反应，接种疫苗后。这是否反映了对感染的更大抵抗力或耐受性尚不清楚，但对疫苗接种产生积极反应的能力表明 SF-pCD 猪是一种适合猪病研究的替代模型。
