Hawrylowicz C M, Duncan L M, Fuhlbrigge R C, Unanue E R
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
J Immunol. 1989 May 15;142(10):3361-8.
Murine splenic B cells did not constitutively express IL-1 activity. After culture with anti-Ig and T cell-conditioned media and then fixation, B cells expressed membrane IL-1 and were able to stimulate growth of the IL-1-dependent T cell clone D10. Expression of membrane IL-1 required stimulation of B cells for 2 days before fixation. Significant IL-1 activity was detectable in freeze-thaw lysates of identical B cell preparations by 12 h. B cells also released IL-1 into the culture media. In situ hybridization studies by using probes to murine IL-1 alpha and IL-1 beta genes supported these observations. Thus, messenger RNA for IL-1 alpha and IL-1 beta rose in parallel, were detected between 6 and 24 h of culture, and declined to low levels by 30 h. Despite the presence of mRNA for IL-1 alpha and IL-1 beta, only IL-1 alpha had functional activity as determined by the use of a mAb to IL-1 alpha. IL-2 was found to be an essential component of the T cell-derived supernatant. Although IL-4 or TNF did not induce significant B cell IL-1 expression, they both caused a modest, but reproducible enhancement when added in combination with IL-2. IFN-gamma, by contrast, partially inhibited IL-1 induction.
小鼠脾脏B细胞不组成性表达IL-1活性。在用抗Ig和T细胞条件培养基培养然后固定后，B细胞表达膜IL-1并能够刺激IL-1依赖性T细胞克隆D10的生长。膜IL-1的表达需要在固定前对B细胞刺激2天。在相同B细胞制剂的冻融裂解物中，12小时后可检测到显著的IL-1活性。B细胞也将IL-1释放到培养基中。使用针对小鼠IL-1α和IL-1β基因的探针进行的原位杂交研究支持了这些观察结果。因此，IL-1α和IL-1β的信使RNA平行上升，在培养6至24小时之间被检测到，并在30小时时降至低水平。尽管存在IL-1α和IL-1β的mRNA，但通过使用抗IL-1α单克隆抗体确定只有IL-1α具有功能活性。发现IL-2是T细胞衍生上清液的必需成分。虽然IL-4或TNF不诱导显著的B细胞IL-1表达，但当与IL-2联合添加时，它们都会引起适度但可重复的增强。相比之下，IFN-γ部分抑制IL-1的诱导。
