Maurice Sundy, Skrede Inger, LeFloch Gaetan, Barbier Georges, Kauserud Håvard
Université de Brest, UEB, EA3882 Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IFR148 ScInBioS, ESMISAB, Technopôle de Brest Iroise, 29280 Plouzané, France, and Microbial Evolution Research Group (MERG), Department of Biosciences, University of Oslo, PO Box 1066 Blindern, N-0316 Oslo, Norway
Microbial Evolution Research Group (MERG), Department of Biosciences, University of Oslo, PO Box 1066 Blindern, N-0316 Oslo, Norway.
Mycologia. 2014 Sep-Oct;106(5):889-95. doi: 10.3852/13-344.
In this study the genetic variation and population structure in a French population of the dry rot fungus S. lacrymans was investigated using 14 microsatellites markers and compared to the rest of Europe. In that comparison the French population possessed the same allelic diversity as rest of Europe. A weak geographic structuring of the genetic variation was observed across Europe, where the French isolates to some extent separated from the rest of Europe, indicating that weak barriers to gene flow exists. Eighty percent of the isolates had unique multilocus microsatellite genotypes, which corresponds to high recombination and dispersal by sexual spores. Deviations from Hardy-Weinberg expectations were observed in multiple loci. In most loci there was an excess of heterozygotes, which could be due to either non-random mating, presence of more than two nuclei in the secondary mycelia or another unrecognized process. A total of six vegetative compatibility (VC) groups were present in Europe, out of which four were sampled in France. One VC group was over-represented in France while two others were underrepresented, as compared to the rest of Europe.
在本研究中，利用14个微卫星标记对法国干腐病菌S. lacrymans种群的遗传变异和种群结构进行了调查，并与欧洲其他地区进行了比较。在该比较中，法国种群拥有与欧洲其他地区相同的等位基因多样性。在欧洲观察到遗传变异存在微弱的地理结构，法国分离株在一定程度上与欧洲其他地区分离，这表明存在微弱的基因流动障碍。80%的分离株具有独特的多位点微卫星基因型，这对应于通过有性孢子进行的高重组和扩散。在多个位点观察到偏离哈迪-温伯格预期的情况。在大多数位点，杂合子过多，这可能是由于非随机交配、次生菌丝体中存在两个以上细胞核或另一个未被认识的过程。欧洲总共存在六个营养体亲和性（VC）组，其中四个在法国进行了采样。与欧洲其他地区相比，一个VC组在法国的比例过高，而另外两个则过低。
