Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université Européenne de Bretagne, ESMISAB, Technopole Brest-Iroise, Plouzané, France.
J Microbiol Methods. 2011 Feb;84(2):208-15. doi: 10.1016/j.mimet.2010.11.018. Epub 2010 Dec 10.
Early detection of indoor wood-decay fungi is crucial to prevent building deterioration and thereby avoid considerable economic loss. Due to their increased sensitivity, two reliable DNA-based fingerprinting techniques, capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) and denaturing high-performance liquid chromatography (DHPLC), were used to identify Serpula lacrymans and to profile wood-rot Basidiomycetes in the built environment. Molecular fungal diversity was assessed on 74 environmental samples, collected from 2003 to 2009 from infected buildings in France. S. lacrymans, the most widespread, indoor wood-decay fungus accounted for 64% of total wood-rot Basidiomycetes. A number of other common wood-rot fungi such as Coniophora puteana, Trametes versicolor and Donkioporia expansa were identified. Other Basidiomycetes such as Phlebiopsis gigantea and Scleroderma verrucosum were detected for the first time in the built environment. Reliable diagnostic tools were developed using two PCR-based molecular typing techniques, one for routine diagnosis and another one for community inventories. Together they provided useful data for characterising the complexity of wood-decay ecosystems and helped reveal the coexistence of different wood-decay fungi within the same microbiotope.
早期发现室内腐朽真菌对于防止建筑物恶化、避免重大经济损失至关重要。由于其敏感性增加，两种可靠的基于 DNA 的指纹识别技术，即毛细管电泳单链构象多态性（CE-SSCP）和变性高效液相色谱（DHPLC），被用于鉴定裂褶菌并分析建筑环境中的木质腐朽担子菌。从 2003 年至 2009 年，从法国受感染的建筑物中采集了 74 个环境样本，对其进行了分子真菌多样性评估。裂褶菌是分布最广的室内木质腐朽真菌，占总木质腐朽担子菌的 64%。还鉴定出了其他一些常见的木质腐朽真菌，如绒盖牛肝菌、杂色云芝和阔孔栓菌。在建筑环境中首次检测到其他担子菌，如 Phlebiopsis gigantea 和 Scleroderma verrucosum。使用两种基于 PCR 的分子分型技术开发了可靠的诊断工具，一种用于常规诊断，另一种用于群落编目。它们共同为描述木质腐朽生态系统的复杂性提供了有用的数据，并有助于揭示同一微生物区系中不同木质腐朽真菌的共存。
