Franzini Maria, Lorenzoni Valentina, Masotti Silvia, Prontera Concetta, Chiappino Dante, Latta Daniele Della, Daves Massimo, Deluggi Irene, Zuin Massimo, Ferrigno Luigina, Mele Alfonso, Marcucci Fabrizio, Caserta Carmelo A, Surace Pasquale, Messineo Arianna, Turchetti Giuseppe, Passino Claudio, Emdin Michele, Clerico Aldo
Scuola Superiora Sant'Anna, Pisa, Italy; Fondazione Toscana G. Monasterio, Pisa, Italy.
Scuola Superiora Sant'Anna, Pisa, Italy.
Clin Chim Acta. 2015 Jan 1;438:376-81. doi: 10.1016/j.cca.2014.09.010. Epub 2014 Sep 17.
The aim of this study is to determine the 99th upper-reference limit (URL) for cardiac troponin T (cTnT) in Italian apparently healthy subjects.
The reference population was selected from 5 cities: Bolzano (n=290), Milano (CAMELIA-Study, n=287), Montignoso (MEHLP-Study, n=306), Pisa (n=182), and Reggio Calabria (MAREA-Study, n=535). Subjects having cardiac/systemic acute/chronic diseases were excluded. Participants to MEHLP project underwent cardiac imaging investigation. High-sensitive cTnT was measured with Cobas-e411 (Roche Diagnostics).
We enrolled 1600 healthy subjects [54.6% males; age range 10-90years; mean (SD): 36.4 (21.2) years], including 34.6% aged <20years, 54.5% between 20 and 64years, and 10.9% over 65years. In the youngest the 99th URL was 10.9ng/L in males and 6.8ng/L in females; in adults 23.2ng/L and 10.2ng/L; and in elderly 36.8ng/L and 28.6ng/L. After the exclusion of outliers the 99th URL values were significantly decreased (P<0.05) in particular those of the oldest (13.8ng/L and 14ng/L). MEHLP participants were divided in healthy and asymptomatic, according to known cardiovascular risk factors (HDL, LDL, glucose, C-reactive protein): the 99th URL of cTnT values of these subgroups was significantly different (19.5 vs. 22.7, P<0.05).
99th URL of cTnT values was strongly affected by age, gender, selection of subjects and the statistical evaluation of outliers.
本研究的目的是确定意大利表面健康受试者中心肌肌钙蛋白T（cTnT）的第99百分位上限参考值（URL）。
参考人群选自5个城市：博尔扎诺（n = 290）、米兰（CAMELIA研究，n = 287）、蒙蒂尼奥索（MEHLP研究，n = 306）、比萨（n = 182）和雷焦卡拉布里亚（MAREA研究，n = 535）。排除患有心脏/全身性急/慢性疾病的受试者。参与MEHLP项目的受试者接受了心脏成像检查。使用Cobas - e411（罗氏诊断）测量高敏cTnT。
我们纳入了1600名健康受试者[男性占54.6%；年龄范围10 - 90岁；平均（标准差）：36.4（21.2）岁]，其中年龄<20岁的占34.6%，20至64岁的占54.5%，65岁以上的占10.9%。在最年轻的受试者中，男性第99百分位URL为10.9ng/L，女性为6.8ng/L；成年人中分别为23.2ng/L和10.2ng/L；老年人中分别为36.8ng/L和28.6ng/L。排除异常值后，第99百分位URL值显著降低（P<0.05），尤其是最年长者的值（13.8ng/L和14ng/L）。根据已知的心血管危险因素（高密度脂蛋白、低密度脂蛋白、血糖、C反应蛋白），将MEHLP参与者分为健康和无症状两组：这些亚组的cTnT值第99百分位URL显著不同（19.5对22.7，P<0.05）。
cTnT值的第99百分位URL受年龄、性别、受试者选择和异常值统计评估的强烈影响。
