Zakaryan Hovakim, Karalova Elena, Voskanyan Henrik, Ter-Pogossyan Zarine, Nersisyan Narek, Hakobyan Astghik, Saroyan David, Karalyan Zaven
Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, P.O. Box 0014, Yerevan, Armenia.
Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, P.O. Box 0014, Yerevan, Armenia; Scientific Center of Stock Breading and Veterinary RA, P.O. Box 0071, Nubarashen Sarahat 2, Yerevan, Armenia.
Vet Microbiol. 2014 Nov 7;174(1-2):223-8. doi: 10.1016/j.vetmic.2014.08.029. Epub 2014 Sep 6.
African swine fever is a highly contagious hemorrhagic disease of pigs caused by African swine fever virus (ASFV). Hemorrhages are the most frequently reported lesions in acute and subacute forms of ASF. Hemorrhagic lesions are accompanied by impaired hemostasis, which includes thrombocytopenia and changes in the coagulation system. In the present study, experimental infection was conducted to elucidate whether a highly virulent ASFV genotype II circulating in the Trans-Caucasus and Eastern Europe affects the hemostasis of infected pigs. Platelet count changes and platelet size, as well as coagulation parameters were evaluated upon experimental infection. In contrast to other ASFV strains, ASFV genotype II showed a significant decrease in the number of platelets from 3rd dpi onwards. Furthermore, a decrease in platelet size was observed throughout the entire period of experiment. A significant increase in the number of platelet aggregates was observed from the beginning of infection. Unlike other ASFV strains, ASFV genotype II induced a slight shortening of an activated partial thromboplastin time (aPTT) throughout the experiment. Thrombin time (TT) was prolonged from day 5 onwards, whereas no changes in prothrombin time (PT) were found upon infection. The level of d-dimers was permanently higher than in control with a peak on day 3 post-infection. ASFV induced a significant decrease in the level of fibrinogen from day 5 till the end of experiment. Thus, it can be concluded that ASFV genotype II isolated in Armenia affects the hemostasis of infected pigs and causes changes that differ from that of other ASFV strains described previously.
非洲猪瘟是由非洲猪瘟病毒（ASFV）引起的猪的一种高度传染性出血性疾病。出血是急性和亚急性非洲猪瘟最常见的病变。出血性病变伴有止血功能受损，包括血小板减少和凝血系统变化。在本研究中，进行了实验性感染，以阐明在跨高加索地区和东欧传播的高致病性ASFV基因型II是否会影响感染猪的止血功能。在实验性感染后评估血小板计数变化、血小板大小以及凝血参数。与其他ASFV毒株不同，ASFV基因型II从感染后第3天起血小板数量显著减少。此外，在整个实验期间观察到血小板大小减小。从感染开始就观察到血小板聚集体数量显著增加。与其他ASFV毒株不同，ASFV基因型II在整个实验过程中导致活化部分凝血活酶时间（aPTT）略有缩短。凝血酶时间（TT）从第5天起延长，而感染后凝血酶原时间（PT）未发现变化。d - 二聚体水平始终高于对照组，在感染后第3天达到峰值。从第5天到实验结束，ASFV导致纤维蛋白原水平显著降低。因此，可以得出结论，在亚美尼亚分离出的ASFV基因型II会影响感染猪的止血功能，并引起与先前描述的其他ASFV毒株不同的变化。
