Liu Yang, Wang Li, Jiang Shanshan, Pan Jiaowen, Cai Guohua, Li Dequan
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China.
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Plant Physiol Biochem. 2014 Nov;84:22-31. doi: 10.1016/j.plaphy.2014.08.016. Epub 2014 Sep 4.
Group 5 LEA (Late Embryogenesis Abundant) proteins contain a significantly higher proportion of hydrophobic residues but lack significant signature motifs or consensus sequences. This group is considered as an atypical group of LEA proteins. Up to now, there is little known about group 5C LEA proteins in maize. Here, we identified a novel group 5C LEA protein from maize. The accumulation of transcripts demonstrated that ZmLEA5C displayed similar induced characteristics in leaves and roots. Transcription of ZmLEA5C could be induced by low temperature, osmotic and oxidative stress and some signaling molecules, such as abscisic acid (ABA), salicylic acid (SA) and methyl jasmonate (MeJA). However, transcription of ZmLEA5C was significantly inhibited by high salinity. Further study indicated that the ZmLEA5C protein could be phosphorylated by the protein kinase CKII. ZmLEA5C could protect the activity of LDH under water deficit and low temperature stresses. Overexpression of ZmLEA5C conferred to transgenic tobacco (Nicotiana benthamiana) and yeast (GS115) tolerance to osmotic and low temperature stresses.
第5组胚胎后期丰富（LEA）蛋白含有显著更高比例的疏水残基，但缺乏显著的特征基序或共有序列。该组被认为是LEA蛋白中的一个非典型组。到目前为止，关于玉米中的第5C组LEA蛋白知之甚少。在此，我们从玉米中鉴定出一种新型的第5C组LEA蛋白。转录本的积累表明ZmLEA5C在叶片和根中表现出相似的诱导特征。ZmLEA5C的转录可被低温、渗透和氧化胁迫以及一些信号分子诱导，如脱落酸（ABA）、水杨酸（SA）和茉莉酸甲酯（MeJA）。然而，高盐显著抑制ZmLEA5C的转录。进一步研究表明，ZmLEA5C蛋白可被蛋白激酶CKII磷酸化。ZmLEA5C在水分亏缺和低温胁迫下可保护乳酸脱氢酶（LDH）的活性。ZmLEA5C的过表达赋予转基因烟草（本氏烟草）和酵母（GS115）对渗透和低温胁迫的耐受性。
Zotero 插件