Su Yating, Fang Jieyun, Zeeshan Ul Haq Muhammad, Yang Wanli, Yu Jing, Yang Dongmei, Liu Ya, Wu Yougen
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.
Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Hainan University, Haikou 570228, China.
Plants (Basel). 2024 Jul 26;13(15):2077. doi: 10.3390/plants13152077.
Casparian strip membrane domain proteins (CASPs), regulating the formation of Casparian strips in plants, serve crucial functions in facilitating plant growth, development, and resilience to abiotic stress. However, little research has focused on the characteristics and functions of in cultivated peanuts. In this study, the genome-wide identification and expression analysis of the gene family was performed using bioinformatics and transcriptome data. Results showed that a total of 80 members on 20 chromosomes were identified and divided into three subclusters, which mainly localized to the cell membrane. Ka/Ks analysis revealed that most of the genes underwent purifying selection. Analysis of elements suggested the possible involvement of in hormonal and stress responses, including GA, MeJA, IAA, ABA, drought, and low temperature. Moreover, 20 different miRNAs for 37 different genes were identified by the psRNATarget service. Likewise, transcriptional analysis revealed key responding to various stresses, hormonal processing, and tissue types, including 33 genes in low temperature and drought stress and 41 genes in tissue-specific expression. These results provide an important theoretical basis for the functions of in growth, development, and multiple stress resistance in cultivated peanuts.
凯氏带膜结构域蛋白（CASPs）在植物中调节凯氏带的形成，在促进植物生长、发育以及对非生物胁迫的抗性方面发挥着关键作用。然而，针对栽培花生中该蛋白的特性和功能的研究较少。在本研究中，利用生物信息学和转录组数据对该基因家族进行了全基因组鉴定和表达分析。结果表明，在20条染色体上共鉴定出80个该蛋白成员，并分为三个亚簇，它们主要定位于细胞膜。Ka/Ks分析表明，大多数基因经历了纯化选择。对顺式作用元件的分析表明，该蛋白可能参与激素和胁迫反应，包括赤霉素（GA）、茉莉酸甲酯（MeJA）、生长素（IAA）、脱落酸（ABA）、干旱和低温。此外，通过psRNATarget服务鉴定出针对37个不同该蛋白基因的20种不同的微小RNA（miRNA）。同样，转录分析揭示了该蛋白对各种胁迫、激素处理和组织类型的关键响应，包括低温和干旱胁迫下的33个基因以及组织特异性表达中的41个基因。这些结果为该蛋白在栽培花生生长、发育和多重抗逆性中的功能提供了重要的理论依据。
