Somani S J, Badgujar L B, Sutariya B K, Saraf M N
Indian J Exp Biol. 2014 Sep;52(9):876-81.
The inflammatory bowel disease (IBD) is an idiopathic, immune mediated and chronic inflammation of the intestine. The study aimed to elucidate the ameliorative effect of methanolic extract of Dillenia indica (DIME), hexane fraction (HFDI) and chloroform fraction (CFDI) of Dillenia indica in acetic acid induced experimental colitis in mice. Macroscopic score, colon weight, colonic catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), myeloperoxidase (MPO), malondialdehyde (MDA), tumor necrosis factor (TNF-alpha), and histological changes were recorded after the treatment regimen of 7 days. Intra-rectal instillation of acetic acid caused enhanced macroscopic score, colon weight, colonic MPO, MDA, and TNF-alpha level. It caused significant decreased level of CAT, SOD and GSH. DIME (800 mg/kg), HFDI (200 mg/kg) and CFDI (200 mg/kg) treatment exhibited significant effect in lowering macroscopic score, colon weight, MPO, MDA, TNF-alpha levels and elevation of CAT, GSH and SOD levels. The results suggest that D. indica has ameliorating effects on experimental colitis by inhibiting the proinflammatory mediators like TNF-alpha production.
炎症性肠病（IBD）是一种特发性、免疫介导的肠道慢性炎症。本研究旨在阐明印度第伦桃甲醇提取物（DIME）、印度第伦桃己烷馏分（HFDI）和氯仿馏分（CFDI）对乙酸诱导的小鼠实验性结肠炎的改善作用。在7天的治疗方案后，记录宏观评分、结肠重量、结肠过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、髓过氧化物酶（MPO）、丙二醛（MDA）、肿瘤坏死因子（TNF-α）以及组织学变化。直肠内注入乙酸导致宏观评分、结肠重量、结肠MPO、MDA和TNF-α水平升高。它导致CAT、SOD和GSH水平显著降低。DIME（800毫克/千克）、HFDI（200毫克/千克）和CFDI（200毫克/千克）治疗在降低宏观评分、结肠重量、MPO、MDA、TNF-α水平以及提高CAT、GSH和SOD水平方面表现出显著效果。结果表明，印度第伦桃通过抑制TNF-α等促炎介质的产生对实验性结肠炎具有改善作用。
