Adjouzem Carine Flore, Gilbert Ateufack, Mbiantcha Marius, Yousseu Nana William, Matah Marthe Mba Vanessa, Djuichou Nguemnang Stephanie Flore, Tsafack Eric Gonzal, Atsamo Albert Donatien
Laboratory of Animal Physiology and Phytopharmacology, Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Laboratory of Animal Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Evid Based Complement Alternat Med. 2020 May 30;2020:4918453. doi: 10.1155/2020/4918453. eCollection 2020.
Among the most exploited species in Cameroon, is widely used in African medicine for the relief of several pathologies including gastrointestinal disorders. This study was conducted in order to assess the effects of aqueous and methanol stem-bark extracts of on DSS- (dextran sodium sulfate-) induced intestinal colitis and to determine its antioxidant potential. The classes of secondary metabolites present in these extracts were determined by chemical screening. The production of TNF-, IL-6, IL-1, and PGE2 was performed by ELISA analysis. Anticolitis effects were determined using an model of ulcerative colitis induced by DSS. The colitis was induced with a double dose of DSS (3% and 1%), and the aqueous and methanol extracts were administered orally from the 6 day after commencement of induction. The phytochemical screening revealed the presence of six classes of secondary metabolites in these crude extracts: tannins, saponins, alkaloids, steroids, flavonoids, and phenols. Methanol and aqueous extracts of significantly ( < 0.001) inhibited TNF-, IL-6, IL-1, and PGE2 production stimulated by LPS. Both extracts at all doses significantly reduced ( < 0.01, < 0.001) the signs of DSS-induced colitis in the Wistar rats by decreasing inflammation and chronic colon damage. In addition, the extracts significantly ( < 0.001) reduced malondialdehyde and nitric oxide levels in the colon and significantly ( < 0.01) increased superoxide dismutase and catalase and reduced glutathione ( < 0.05). Both extracts showed greater activity than the reference substance (prednisolone 4 mg/kg) used in this study. This study has demonstrated that aqueous and methanol extracts of stem bark have healing properties against colitis experimentally induced by DSS in rats.
在喀麦隆被大量开发利用的物种中，[物种名称]在非洲医学中被广泛用于缓解多种病症，包括胃肠道疾病。本研究旨在评估[物种名称]水提取物和甲醇提取物对葡聚糖硫酸钠（DSS）诱导的肠道结肠炎的影响，并确定其抗氧化潜力。通过化学筛选确定这些提取物中存在的次生代谢物类别。采用酶联免疫吸附测定（ELISA）分析检测肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-1（IL-1）和前列腺素E2（PGE2）的产生。使用DSS诱导的溃疡性结肠炎模型确定抗结肠炎作用。用双倍剂量的DSS（3%和1%）诱导结肠炎，并在诱导开始后的第6天开始口服给予水提取物和甲醇提取物。植物化学筛选显示这些粗提取物中存在六类次生代谢物：单宁、皂苷、生物碱、甾体、黄酮类化合物和酚类。[物种名称]的甲醇提取物和水提取物显著（P<0.001）抑制脂多糖（LPS）刺激产生的TNF-α、IL-6、IL-1和PGE2。所有剂量的两种提取物均通过减轻炎症和慢性结肠损伤，显著（P<0.01，P<0.001）减轻Wistar大鼠DSS诱导的结肠炎症状。此外，提取物显著（P<0.001）降低结肠中丙二醛和一氧化氮水平，并显著（P<0.01）提高超氧化物歧化酶、过氧化氢酶水平以及还原型谷胱甘肽水平（P<0.05）。两种提取物均显示出比本研究中使用的参考物质（泼尼松龙4mg/kg）更强的活性。本研究表明，[物种名称]茎皮的水提取物和甲醇提取物对大鼠实验性DSS诱导的结肠炎具有治疗作用。
