[增殖性糖尿病视网膜病变中血浆及眼内组织VEGF表达与t-PA和PAI表达的相关性研究]

[The correlation study of the expression of VEGF with t-PA and PAI expression in plasma and intraocular tissue in proliferative diabetic retinopathy].

作者信息

Wu Shuling, He Xianglian

机构信息

Department of Ophthalmology, General Hospital of Ningxia Medical University, Yinchuan 750004, China. Email:

Department of Ophthalmology, General Hospital of Ningxia Medical University, Yinchuan 750004, China.

出版信息

Zhonghua Yan Ke Za Zhi. 2014 Jun;50(6):448-53.

PMID:25241978

Abstract

OBJECTIVE

To evaluate the correlation between the expression of VEGF with t-PA and PAI expression in plasma aqueous humor and vitreous of proliferative diabetic retinopathy (PDR).

METHODS

It was an experimental study. The concentrations of VEGF t-PA and PAI in plasma, aqueous humor and vitreous body in PDR and normal group were detected by ELISA.

RESULTS

The levels of VEGF (53.37 ng/L) and PAI (8.00 µg/L) in the plasma of PDR patients were higher than control group, and there was significant statistically difference between them (Z = -3.437, -5.816; P < 0.01). The levels of t-PA (24.32 µg/L) in plasma of PDR patients and control group was no statistically difference between them (Z = -1.715, P = 0.086). The levels of VEGF (335.00 ng/L) t-PA (5.70 µg/L) and PAI (0.63 µg/L) in the aqueous humor of PDR patients were higher than control group, and there was statistically difference between them (Z = -4.805, -1.967, -4.018; P < 0.05). The levels of VEGF (691.69 ng/L) t-PA (13.05 µg/L) and PAI (4.01 µg/L) in the vitreous of PDR patients were higher than control group , and there was statistically difference between them (Z = -2.807, -2.642, -2.230;P < 0.05). Compare the plasma aqueous humor and vitreous of PDR patients. The levels of VEGF is: vitreous>aqueous humor>plasma. The levels of t-PA is: plasma>vitreous>aqueous humor. The levels of PAI is: plasma and vitreous>aqueous humor. The expression of VEGF was highly correlated with t-PA and PAI in plasma aqueous humor and vitreous of PDR (P < 0.05).

CONCLUSIONS

VEGF PAI in plasma, VEGF t-PA PAI in aqueous humor and vitreous were increases. The expression of VEGF was positive correlated with t-PA and PAI in plasma aqueous humor and vitreous of PDR.

摘要

目的

评估增殖性糖尿病视网膜病变（PDR）患者血浆、房水和玻璃体中血管内皮生长因子（VEGF）表达与组织型纤溶酶原激活剂（t-PA）及纤溶酶原激活物抑制剂（PAI）表达之间的相关性。

方法

本研究为实验性研究。采用酶联免疫吸附测定法（ELISA）检测PDR组和正常组患者血浆、房水和玻璃体中VEGF、t-PA及PAI的浓度。

结果

PDR患者血浆中VEGF水平（53.37 ng/L）及PAI水平（8.00 μg/L）高于对照组，差异有统计学意义（Z = -3.437，-5.816；P < 0.01）。PDR患者血浆中t-PA水平（24.32 μg/L）与对照组比较，差异无统计学意义（Z = -1.715，P = 0.086）。PDR患者房水中VEGF水平（335.00 ng/L）、t-PA水平（5.70 μg/L）及PAI水平（0.63 μg/L）高于对照组，差异有统计学意义（Z = -4.805，-1.967，-4.018；P < 0.05）。PDR患者玻璃体中VEGF水平（691.69 ng/L）、t-PA水平（13.05 μg/L）及PAI水平（4.01 μg/L）高于对照组，差异有统计学意义（Z = -2.807，-2.642，-2.230；P < 0.05）。比较PDR患者的血浆、房水和玻璃体，VEGF水平为：玻璃体>房水>血浆；t-PA水平为：血浆>玻璃体>房水；PAI水平为：血浆和玻璃体>房水。PDR患者血浆、房水和玻璃体中VEGF的表达与t-PA及PAI高度相关（P < 0.05）。