Murohashi I, Tohda S, Suzuki T, Nagata K, Yamashita Y, Nara N, Aoki N
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Cancer Res. 1989 Jun 15;49(12):3431-5.
We describe here the presence of a single class of interleukin 1 beta (IL-1 beta) receptors on the surface of blast cells freshly obtained from eight acute myeloblastic leukemia patients and one chronic myelocytic leukemia patient in blast crisis. Blast cells possessed a low number of high-affinity receptors (range, less than 10-173 receptors/cell) with a Kd of 1.8-12.8 x 10(-10) M. At the same time, we have investigated the effects of IL-1 on the growth of leukemic blast progenitors, and a significant heterogeneity of responsiveness was observed. IL-1 beta (1 ng/ml) enhanced blast colony formation in six patients. No significant effect was observed by addition of up to 100 ng/ml of IL-1 beta in the remaining three patients. No significant correlation was observed between the receptor number, receptor affinity, and the cellular responsiveness to IL-1; in some acute myeloblastic leukemia cases with apparent IL-1 receptors, no proliferation response to added IL-1 was observed. Our data show that IL-1 alone can enhance blast colony formation and that lack of responsiveness to IL-1 in some acute myeloblastic leukemia patients is not related to the absence of IL-1 receptors on blast cells.
我们在此描述了从8例急性髓细胞白血病患者和1例处于原始细胞危象的慢性粒细胞白血病患者新鲜获取的原始细胞表面存在单一类别的白细胞介素1β（IL-1β）受体。原始细胞具有少量高亲和力受体（范围为每细胞少于10 - 173个受体），解离常数（Kd）为1.8 - 12.8×10⁻¹⁰ M。同时，我们研究了IL-1对白血病原始祖细胞生长的影响，观察到反应性存在显著异质性。IL-1β（1 ng/ml）增强了6例患者的原始细胞集落形成。在其余3例患者中，加入高达100 ng/ml的IL-1β未观察到显著影响。受体数量、受体亲和力与细胞对IL-1的反应性之间未观察到显著相关性；在一些明显具有IL-1受体的急性髓细胞白血病病例中，未观察到对添加的IL-1的增殖反应。我们的数据表明，单独的IL-1可增强原始细胞集落形成，并且一些急性髓细胞白血病患者对IL-1缺乏反应性与原始细胞上不存在IL-1受体无关。
