Whatmore Adrian M, Davison Nicholas, Cloeckaert Axel, Al Dahouk Sascha, Zygmunt Michel S, Brew Simon D, Perrett Lorraine L, Koylass Mark S, Vergnaud Gilles, Quance Christine, Scholz Holger C, Dick Edward J, Hubbard Gene, Schlabritz-Loutsevitch Natalia E
OIE/WHO/FAO Brucellosis Reference Laboratory, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (AHVLA), Woodham Lane, Addlestone KT15 3NB, UK.
Animal Health and Veterinary Laboratories Agency (AHVLA), Polwhele, Truro TR4 9AD, UK.
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4120-4128. doi: 10.1099/ijs.0.065482-0. Epub 2014 Sep 21.
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60(T) and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella. This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus Brucella. The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus Brucella, reacting only with Brucella A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk2 and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO2 and no production of H2S but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus Brucella. Molecular analysis of the omp2 locus genes showed that both strains had a novel combination of two highly similar omp2b gene copies. The two strains shared a unique fingerprint profile of the multiple-copy Brucella-specific element IS711. Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus Brucella. Isolates F8/08-60(T) and F8/08-61 could be distinguished clearly from all known species of the genus Brucella and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus Brucella suggested by the ICSP Subcommittee on the Taxonomy of Brucella, they represent a novel species within the genus Brucella, for which the name Brucella papionis sp. nov. is proposed, with the type strain F8/08-60(T) ( = NCTC 13660(T) = CIRMBP 0958(T)).
从产下死胎的狒狒（Papio spp.）的临床标本中分离出两株革兰氏阴性、无运动性、不形成芽孢的球状细菌（菌株F8/08 - 60(T)和F8/08 - 61），并对其进行了多相分类研究。基于16S rRNA基因序列相似性，这两株具有相同序列的菌株被归入布鲁氏菌属。通过扩展多位点序列分析（MLSA）以及对一株代表性分离株进行全基因组测序，进一步证实了这一归类，在MLSA中两株菌也具有相同序列。上述所有分析表明，这两株菌代表了布鲁氏菌属内的一个新谱系。当采用传统上用于区分布鲁氏菌属各物种的表型分析方法时，这两株菌也呈现出独特的特征，仅与布鲁氏菌A单特异性抗血清发生反应，对染料硫堇和品红敏感，在常规试验稀释度（RTD）下被噬菌体Wb、Bk2和Fi噬菌体裂解，但仅对噬菌体Tb部分敏感，不需要二氧化碳，不产生硫化氢，但具有很强的脲酶活性。生化分析揭示了一种与布鲁氏菌属现有物种不同的酶活性和代谢能力模式。omp2基因座基因的分子分析表明，两株菌都有两个高度相似的omp2b基因拷贝组成的新组合。这两株菌共享多拷贝布鲁氏菌特异性元件IS711的独特指纹图谱。与MLSA一样，多位点可变数目串联重复分析（MLVA）表明，这些分离株紧密聚集在一起，但代表了布鲁氏菌属内的一个独特群体。分离株F8/08 - 60(T)和F8/08 - 61在表型和分子特性上都能与布鲁氏菌属的所有已知物种及其生物变种明显区分开来。因此，根据国际微生物学联合会布鲁氏菌分类学小组委员会提出的布鲁氏菌属物种概念，它们代表了布鲁氏菌属内的一个新物种，为此提议将其命名为布鲁氏狒狒菌（Brucella papionis sp. nov.），模式菌株为F8/08 - 60(T)（=NCTC 13660(T)=CIRMBP 0958(T)）。
