Division of Foodborne, Bacterial, and Mycotic Diseases and Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
BMC Microbiol. 2010 Jan 27;10:23. doi: 10.1186/1471-2180-10-23.
Brucellosis is primarily a zoonotic disease caused by Brucella species. There are currently ten Brucella spp. including the recently identified novel B. inopinata sp. isolated from a wound associated with a breast implant infection. In this study we report on the identification of an unusual Brucella-like strain (BO2) isolated from a lung biopsy in a 52-year-old patient in Australia with a clinical history of chronic destructive pneumonia.
Standard biochemical profiles confirmed that the unusual strain was a member of the Brucella genus and the full-length 16S rRNA gene sequence was 100% identical to the recently identified B. inopinata sp. nov. (type strain BO1(T)). Additional sequence analysis of the recA, omp2a and 2b genes; and multiple locus sequence analysis (MLSA) demonstrated that strain BO2 exhibited significant similarity to the B. inopinata sp. compared to any of the other Brucella or Ochrobactrum species. Genotyping based on multiple-locus variable-number tandem repeat analysis (MLVA) established that the BO2 and BO1(T) strains form a distinct phylogenetic cluster separate from the other Brucella spp.
Based on these molecular and microbiological characterizations, we propose that the BO2 strain is a novel lineage of the newly described B. inopinata species.
布鲁氏菌病主要是一种由布鲁氏菌属引起的人畜共患病。目前有十种布鲁氏菌属，包括最近从与乳房植入物感染相关的伤口中分离出来的新型 B. inopinata sp.。在本研究中，我们报告了从澳大利亚一名 52 岁患者的肺部活检中分离出的一种不寻常的布鲁氏菌样菌株（BO2），该患者有慢性破坏性肺炎的临床病史。
标准生化特征证实，该不寻常菌株是布鲁氏菌属的一个成员，全长 16S rRNA 基因序列与最近鉴定的 B. inopinata sp. nov.（BO1(T)）完全相同。recA、omp2a 和 2b 基因的额外序列分析和多位点序列分析（MLSA）表明，与其他布鲁氏菌或 Ochrobactrum 种相比，BO2 菌株与 B. inopinata sp. 具有显著相似性。基于多位点可变数串联重复分析（MLVA）的基因分型表明，BO2 和 BO1(T)菌株形成了一个独特的进化枝，与其他布鲁氏菌属分离。
基于这些分子和微生物学特征，我们提出 BO2 菌株是新描述的 B. inopinata 种的一个新谱系。
