Yee G K, Ullrich S E, Kripke M L
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston.
Cell Immunol. 1989 Jun;121(1):88-98. doi: 10.1016/0008-8749(89)90007-5.
The purpose of this study was to determine whether multiple types of suppressor factors play a role in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes (UV Ts). The UV Ts were induced by applying contact allergens to the ventral, unirradiated skin of mice exposed 5 days earlier to UVB radiation. Previous studies indicated that supernatants from cultures containing UV Ts, normal lymphocytes, and hapten-modified cells suppressed contact hypersensitivity (CHS) in vivo and cytotoxic T lymphocyte (CTL) generation in vitro in a hapten-specific manner. In this report, cell-free lysates from sonically disrupted UV Ts were examined for their ability to suppress these responses. When lysates were injected into normal animals at the time of sensitization, they inhibited CHS in a hapten-nonspecific manner. In addition, the lysates suppressed not only the induction but also the elicitation of CHS, and they suppressed the generation of CTL. Lysates prepared from spleen cells obtained from non-UV-irradiated mice or UV-irradiated, unsensitized mice failed to inhibit either response. Moreover, in contrast to the lysates, the hapten-specific UV Ts culture supernatants inhibited the induction but not the elicitation of CHS. These results suggest that both hapten-specific and nonspecific inhibitory factors may participate in the regulation of immune responses by UV Ts.
本研究的目的是确定多种类型的抑制因子是否在紫外线辐射诱导的抑制性T淋巴细胞（UV Ts）对免疫反应的调节中发挥作用。UV Ts是通过将接触性变应原应用于5天前暴露于UVB辐射的小鼠未照射的腹部皮肤而诱导产生的。先前的研究表明，含有UV Ts、正常淋巴细胞和半抗原修饰细胞的培养物上清液以半抗原特异性方式在体内抑制接触性超敏反应（CHS），在体外抑制细胞毒性T淋巴细胞（CTL）的产生。在本报告中，检测了经超声破碎的UV Ts的无细胞裂解物抑制这些反应的能力。当在致敏时将裂解物注射到正常动物体内时，它们以半抗原非特异性方式抑制CHS。此外，裂解物不仅抑制CHS的诱导，还抑制其激发，并且它们抑制CTL的产生。从未接受紫外线照射的小鼠或接受紫外线照射但未致敏的小鼠获得的脾细胞制备的裂解物未能抑制任何一种反应。此外，与裂解物相反，半抗原特异性的UV Ts培养上清液抑制CHS的诱导但不抑制其激发。这些结果表明，半抗原特异性和非特异性抑制因子可能都参与了UV Ts对免疫反应的调节。
