Ullrich S E
Immunology. 1985 Feb;54(2):343-52.
Application of a contact-sensitizing agent to the skin of mice previously exposed to UV radiation at a different site results in the induction of hapten-specific suppressor T lymphocytes. When splenic lymphocytes from such mice were cultured with normal lymphocytes and hapten-conjugated splenic adherent cells, the primary proliferative response was suppressed. The cell responsible for the suppression in vitro was a T lymphocyte, and two signals were required for its induction, ultraviolet radiation and hapten sensitization. The T cell suppressing lymphoproliferation was specific for the hapten applied after UV radiation. The UV-induced T suppressor cell inhibited only primary lymphoproliferation; the response of lymphocytes from immunized mice was unaffected. The activity of the UV-induced suppressor cell was not affected by mitomycin C treatment. Thus, suppression of the primary proliferative response of lymphocytes to hapten-modified syngeneic cells in vitro correlates with in vivo suppression of contact hypersensitivity by these UV-induced suppressor cells. This suggests that the suppressor cells act by preventing the proliferation of hapten-specific responder clones. Use of this in vitro assay system should facilitate investigation of the characteristics of these cells and the mechanism by which these regulatory T lymphocytes inhibit contact sensitization.
将接触致敏剂应用于先前在不同部位接受过紫外线辐射的小鼠皮肤，可诱导产生半抗原特异性抑制性T淋巴细胞。当将此类小鼠的脾淋巴细胞与正常淋巴细胞以及半抗原结合的脾黏附细胞共同培养时，原发性增殖反应受到抑制。体外负责抑制作用的细胞是T淋巴细胞，其诱导需要两个信号，即紫外线辐射和半抗原致敏。抑制淋巴细胞增殖的T细胞对半抗原具有特异性，该半抗原是在紫外线辐射后应用的。紫外线诱导的T抑制细胞仅抑制原发性淋巴细胞增殖；免疫小鼠的淋巴细胞反应不受影响。紫外线诱导的抑制细胞的活性不受丝裂霉素C处理的影响。因此，体外淋巴细胞对半抗原修饰的同基因细胞的原发性增殖反应的抑制与这些紫外线诱导的抑制细胞在体内对接触性超敏反应的抑制相关。这表明抑制细胞通过阻止半抗原特异性反应性克隆的增殖起作用。使用这种体外检测系统应有助于研究这些细胞的特性以及这些调节性T淋巴细胞抑制接触致敏的机制。
