Dušanić Daliborka, Benčina Dušan, Narat Mojca, Oven Irena
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domzale, Slovenia.
Biomed Res Int. 2014;2014:613730. doi: 10.1155/2014/613730. Epub 2014 Aug 26.
In infectious synovitis caused by Mycoplasma synoviae chicken chondrocytes (CCH) may come into direct contact with these bacteria that are also capable of invading CCH in vitro. In this study, phenotype microarrays were used to evaluate the influence of Mycoplasma synoviae on the global metabolic activity of CCH. Therefore, CCH were cultured in the presence of 504 individual compounds, spotted in wells of 11 phenotype microarrays for eukaryotic cells, and exposed to Mycoplasma synoviae membranes or viable Mycoplasma synoviae. Metabolic activity and sensitivity of normal cells versus infected cells were evaluated. Metabolic profiles of CCH treated with viable Mycoplasma synoviae or its membranes were significantly different from those of CCH alone. CCH treated with Mycoplasma synoviae membranes were able to use 48 carbon/nitrogen sources not used by CCH alone. Treatment also influenced ion uptake in CCH and intensified the sensitivity to 13 hormones, 5 immune mediators, and 29 cytotoxic chemicals. CCH were even more sensitive to hormones/immune mediators when exposed to viable Mycoplasma synoviae. Our results indicate that exposure to Mycoplasma synoviae or its membranes induces a wide range of metabolic and sensitivity modifications in CCH that can contribute to pathological processes in the development of infectious synovitis.
在由滑液支原体引起的传染性滑膜炎中，鸡软骨细胞（CCH）可能会直接接触这些细菌，并且这些细菌在体外也能够侵入CCH。在本研究中，使用表型芯片来评估滑液支原体对CCH整体代谢活性的影响。因此，将CCH培养在存在504种单独化合物的环境中，这些化合物点样于11个真核细胞表型芯片的孔中，并使其暴露于滑液支原体膜或活的滑液支原体中。评估正常细胞与感染细胞的代谢活性和敏感性。用活的滑液支原体或其膜处理的CCH的代谢谱与单独的CCH有显著差异。用滑液支原体膜处理的CCH能够利用48种单独的CCH未使用的碳/氮源。处理还影响了CCH中的离子摄取，并增强了对13种激素、5种免疫介质和29种细胞毒性化学物质的敏感性。当暴露于活的滑液支原体时，CCH对激素/免疫介质更为敏感。我们的结果表明，暴露于滑液支原体或其膜会在CCH中诱导广泛的代谢和敏感性改变，这可能有助于传染性滑膜炎发展过程中的病理过程。
