Curciarello Renata, Steele Alison, Cooper Dianne, MacDonald Thomas T, Kruidenier Laurens, Kudo Takahiro
Centre for Immunology and Infectious Disease, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.
William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.
PLoS One. 2014 Sep 22;9(9):e107933. doi: 10.1371/journal.pone.0107933. eCollection 2014.
Despite their abundance at gastrointestinal sites, little is known about the role of galectins in gut immune responses. We have therefore investigated the Citrobacter rodentium model of colonic infection and inflammation in Galectin-1 or Galectin-3 null mice. Gal-3 null mice showed a slight delay in colonisation after inoculation with C. rodentium and a slight delay in resolution of infection, associated with delayed T cell, macrophage and dendritic cell infiltration into the gut mucosa. However, Gal-1 null mice also demonstrated reduced T cell and macrophage responses to infection. Despite the reduced T cell and macrophage response in Gal-1 null mice, there was no effect on C. rodentium infection kinetics and pathology. Overall, Gal-1 and Gal-3 play only a minor role in immunity to a gut bacterial pathogen.
尽管半乳糖凝集素在胃肠道部位大量存在，但关于其在肠道免疫反应中的作用却知之甚少。因此，我们研究了半乳糖凝集素-1或半乳糖凝集素-3基因敲除小鼠的结肠感染和炎症的鼠柠檬酸杆菌模型。半乳糖凝集素-3基因敲除小鼠在接种鼠柠檬酸杆菌后，其定殖过程略有延迟，感染的消退也稍有延迟，这与T细胞、巨噬细胞和树突状细胞向肠道黏膜的浸润延迟有关。然而，半乳糖凝集素-1基因敲除小鼠对感染的T细胞和巨噬细胞反应也有所降低。尽管半乳糖凝集素-1基因敲除小鼠的T细胞和巨噬细胞反应降低，但对鼠柠檬酸杆菌的感染动力学和病理学没有影响。总体而言，半乳糖凝集素-1和半乳糖凝集素-3在对肠道细菌病原体的免疫中仅起次要作用。
