Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, room C-221, Brasília, Distrito Federal, 70.790-160, Brazil.
Clin Oral Investig. 2021 May;25(5):3285-3295. doi: 10.1007/s00784-020-03660-3. Epub 2020 Nov 7.
The present study aimed to identify proteins obtained from pulp tissue and correlate with each clinical diagnosis (healthy pulp, inflamed pulp, and necrotic pulp).
A total of forty-five molars were used. Three biological replicas were evaluated. Lysis and sonication were used for protein extraction. Protein quantification was assessed by using the Bradford technique, and shotgun proteome analysis was performed by nanoUPLC-MS using a Synapt G2 mass spectrometer. Mass spectra data were processed using the Waters PLGS software, and protein identification was done using the human Uniprot database appended to the PLGS search engine.
A total of 123 different proteins were identified in all evaluated pulp conditions. Among these, 66 proteins were observed for healthy pulp, 66 for inflamed pulp, and 91 for necrotic pulp. Most protein identification was related to immune response, multi-organism process, platelet activation, and stress in inflamed pulp samples compared to healthy pulp. Proteins related to cellular component organization or biogenesis, developmental process, growth, immune response, multi-organism process, response to stimulus, signaling, stress, and transport were identified in cases of apical periodontitis compared to inflamed pulp.
The progression of the disease to inflamed pulp promoted a high abundance of proteins related to the immune system and stress. Comparing the necrotic pulp with inflamed pulp conditions, a high abundance of proteins was noticed related to metabolism, transport, and response between organisms.
This finding may assist in future studies of new markers, understanding of tissue engineering, and development of future products.
本研究旨在鉴定从牙髓组织中获得的蛋白质，并将其与每个临床诊断（健康牙髓、发炎牙髓和坏死牙髓）相关联。
共使用了 45 颗磨牙。评估了三个生物学复制品。使用裂解和超声处理进行蛋白质提取。使用 Bradford 技术评估蛋白质定量，并用 Synapt G2 质谱仪进行纳升超高效液相色谱 - 质谱分析。使用 Waters PLGS 软件处理质谱数据，并使用人类 Uniprot 数据库和 PLGS 搜索引擎进行蛋白质鉴定。
在所有评估的牙髓条件下共鉴定出 123 种不同的蛋白质。其中，健康牙髓有 66 种蛋白质，发炎牙髓有 66 种蛋白质，坏死牙髓有 91 种蛋白质。与健康牙髓相比，在发炎牙髓样本中观察到大多数蛋白质与免疫反应、多器官过程、血小板激活和应激有关。与细胞成分组织或生物发生、发育过程、生长、免疫反应、多器官过程、对刺激的反应、信号转导、应激和运输有关的蛋白质在根尖周炎病例中与发炎牙髓相比被鉴定出来。
疾病向发炎牙髓的进展促进了大量与免疫系统和应激相关的蛋白质的产生。将坏死牙髓与发炎牙髓条件进行比较，发现大量与代谢、运输和生物体之间的反应有关的蛋白质。
这一发现可能有助于未来对新标志物的研究、对组织工程的理解以及未来产品的开发。
