Topoll H H, Zwadlo G, Lange D E, Sorg C
J Periodontal Res. 1989 Mar;24(2):106-12. doi: 10.1111/j.1600-0765.1989.tb00864.x.
The purpose of the present study was to investigate whether functionally different macrophages are present in clinically healthy gingiva and during human experimental gingivitis. Eight male probands were introduced to an oral hygiene program until all reached mean Plaque and Gingival Index scores approaching zero. During the following 19 days all oral hygiene was abandoned. At d -14, 0, 2, 4, 7, 11 and 19 clinical indices and gingival biopsies were taken. Cryostat sections were incubated with monoclonal antibodies against mature macrophages (25F9), inflammatory macrophages (27E10) and anti-inflammatory macrophages (RM 3/1). Positive cells were counted in the inflammatory infiltrate (IF) and the connective tissue (CT). At d -14 elevated numbers of 27E10-positive cells were observed which decreased significantly at d 0 (p less than 0.018) and increased again at d 19 (p less than 0.026). Significant differences in the number of RM 3/1-positive cells were found between d 0 and d -14, 2, 4 and 7 (p less than 0.05) while no differences in the number of 25F9-positive cells were observed throughout this study. It was concluded that experimental gingival inflammation is characterized by the appearance and disappearance of functionally different macrophage subpopulations.
本研究的目的是调查在临床健康牙龈以及人类实验性牙龈炎期间是否存在功能不同的巨噬细胞。八名男性受试者参与了一项口腔卫生计划，直到所有人的菌斑和牙龈指数平均得分接近零。在接下来的19天里，所有口腔卫生措施都被放弃。在第-14、0、2、4、7、11和19天采集临床指标和牙龈活检样本。冰冻切片与针对成熟巨噬细胞（25F9）、炎性巨噬细胞（27E10）和抗炎巨噬细胞（RM 3/1）的单克隆抗体孵育。在炎性浸润（IF）和结缔组织（CT）中对阳性细胞进行计数。在第-14天观察到27E10阳性细胞数量增加，在第0天显著减少（p<0.018），并在第19天再次增加（p<0.026）。在第0天与第-14、2、4和7天之间，RM 3/1阳性细胞数量存在显著差异（p<0.05），而在整个研究过程中未观察到25F9阳性细胞数量的差异。得出的结论是，实验性牙龈炎症的特征是功能不同的巨噬细胞亚群的出现和消失。
