与逆转录病毒载体几乎没有核苷酸序列同源性的新型逆转录病毒辅助细胞。
New retrovirus helper cells with almost no nucleotide sequence homology to retrovirus vectors.
作者信息
Dougherty J P, Wisniewski R, Yang S L, Rhode B W, Temin H M
机构信息
McArdle Laboratory, University of Wisconsin, Madison 53706.
出版信息
J Virol. 1989 Jul;63(7):3209-12. doi: 10.1128/JVI.63.7.3209-3212.1989.
Abstract
We prepared retrovirus packaging cell lines containing gag-pol genes from spleen necrosis virus (expressed from a cytomegalovirus promoter and the simian virus 40 (SV40) polyadenylation sequences) and, on a separate vector, either the env gene from spleen necrosis virus (expressed from the Rous sarcoma virus promoter and the SV40 polyadenylation sequences) or the env gene from amphotropic murine leukemia virus (expressed from a cytomegalovirus promoter and the SV40 polyadenylation sequences). The nucleotide sequences in these packaging cell lines have almost no homology to the retrovirus vectors we used. Retrovirus vectors were produced from these new helper cell lines without any genetic interactions between the vectors and sequences in the helper cells and without transfer of the packaging sequences.
摘要
我们制备了逆转录病毒包装细胞系，其中包含来自脾坏死病毒的gag-pol基因（由巨细胞病毒启动子和猿猴病毒40（SV40）聚腺苷酸化序列表达），并且在一个单独的载体上，要么是来自脾坏死病毒的env基因（由劳氏肉瘤病毒启动子和SV40聚腺苷酸化序列表达），要么是来自嗜异性小鼠白血病病毒的env基因（由巨细胞病毒启动子和SV40聚腺苷酸化序列表达）。这些包装细胞系中的核苷酸序列与我们使用的逆转录病毒载体几乎没有同源性。逆转录病毒载体由这些新的辅助细胞系产生，载体与辅助细胞中的序列之间没有任何遗传相互作用，并且没有包装序列的转移。
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High-frequency deletion in recovered retrovirus vectors containing exogenous DNA with promoters.在含有带启动子的外源DNA的回收逆转录病毒载体中的高频缺失。
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Encapsidation sequences for spleen necrosis virus, an avian retrovirus, are between the 5' long terminal repeat and the start of the gag gene.禽逆转录病毒脾坏死病毒的包装序列位于5'长末端重复序列和gag基因起始位点之间。
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