Parvaz P, Mathian B, Patricot M C, Garcia I, Revol A, Mappus E, Grenot C, Cuilleron C Y
Laboratoire de Biochimie, Hôpital Ste. Eugènie, Centre Hospitalier, Lyon-Sud, Pierre-Bénite, France.
J Steroid Biochem. 1989 Apr;32(4):553-8. doi: 10.1016/0022-4731(89)90389-0.
Monoclonal antibodies with a much higher specificity for DHA-S than for DHA were obtained from a BALB/c mouse immunized with a non-sulphated DHA-7CMO-BSA antigen. An improved fusion technique using PEG containing 10% DMSO instead of PEG alone increased the number of positive hybridomas. One of the five monoclonal antibodies obtained, showed a high affinity for DHA-S (Ka = 10(10) M-1) and very low cross-reactions with androsterone (0.62%) and androsterone sulphate (0.83%) which made it potentially useful for direct quantitation of DHA-S in human serum.
用非硫酸化的DHA-7CMO-BSA抗原免疫BALB/c小鼠，获得了对DHA-S的特异性比对DHA高得多的单克隆抗体。使用含10%二甲基亚砜的聚乙二醇而非单独使用聚乙二醇的改进融合技术增加了阳性杂交瘤的数量。所获得的五种单克隆抗体之一对DHA-S表现出高亲和力（Ka = 10(10) M-1），与雄甾酮（0.62%）和硫酸雄甾酮（0.83%）的交叉反应非常低，这使其有可能用于直接定量人血清中的DHA-S。
