Narasimha Rao P, Moore P H
Steroids. 1976 Jul;28(1):110-3. doi: 10.1016/0039-128x(76)90130-6.
Antiserum for radioimmunoassay (RIA) of dehydroepiandrosterone (DHA) was raised in rabbits with the conjugate obtained by coupling 15beta-carboxyethylmercapto-dehydroepiandrosterone with bovine serum albumin. The antiserum proved to have high affinity and specificity for DHA and showed only minor cross-reaction to androst-5-ene-3beta, 17beta-diol (3.5%). The Rivanol-treated antiserum was covalently coupled to Enzacryl Polyacetal to evaluate its utility in solid-phase RIA.
用15β-羧乙基巯基脱氢表雄酮与牛血清白蛋白偶联得到的结合物免疫家兔，制备了用于脱氢表雄酮（DHA）放射免疫分析（RIA）的抗血清。该抗血清对DHA具有高亲和力和特异性，对雄甾-5-烯-3β,17β-二醇的交叉反应仅为3.5%。用利凡诺处理后的抗血清与恩扎克里尔聚缩醛共价偶联，以评估其在固相RIA中的实用性。
