采血过程中使用引流袋时细菌污染的发生率：马来西亚一项单中心研究
Prevalence of Bacterial Contamination when using a Diversion Pouch during Blood Collection: A Single Center Study in Malaysia.
作者信息
Jumaah Norlaili, Joshi Sanmukh Ratilal, Sandai Doblin
机构信息
Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam 13200 Kepala Batas, Penang, Malaysia.
出版信息
Malays J Med Sci. 2014 May;21(3):47-53.
BACKGROUND
The implementation of diversion pouches is to minimise the risk of bacterial contamination as the initial blood flow is prevented from entering primary bag collections as it is diverted into a pouch. This study was carried out to determine the prevalence of bacterial contamination in the diversion pouches used during blood collections in the Transfusion Department of Hospital Seberang Jaya, Penang, Malaysia.
METHODS
BD Bactec™ Fx instrument detection system was performed on 702 samples of 20 mL of diverting blood in diversion pouch. The inocullum volume was 10 mL for both aerobic and anaerobic bottles cultures and incubated for 5 days in the BD Bactec™ Fx instrument. Positive sample was flagged by BD Bactec™ Fx instrument and subculture to identify the species of organism.
RESULTS
The results showed that of 702 samples, 12 (1.7%) were contaminated. The bacterial species identified were coagulase negative Staphylococcus, Staphylococcus aureus and Gram positive Bacilli.
CONCLUSION
The results strongly suggest that the usage of diversion pouch is of significant importance in reducing bacterial contamination during blood collection.
背景
使用分流袋的目的是将细菌污染风险降至最低，因为初始血流在分流至袋子时不会进入主袋收集器。本研究旨在确定马来西亚槟城梳邦再也医院输血科采血过程中使用的分流袋中细菌污染的发生率。
方法
使用BD Bactec™ Fx仪器检测系统对702份20 mL分流袋中分流血液的样本进行检测。需氧瓶和厌氧瓶培养的接种量均为10 mL，并在BD Bactec™ Fx仪器中培养5天。阳性样本由BD Bactec™ Fx仪器标记，并进行传代培养以鉴定生物体种类。
结果
结果显示，在702份样本中，有12份（1.7%）被污染。鉴定出的细菌种类为凝固酶阴性葡萄球菌、金黄色葡萄球菌和革兰氏阳性杆菌。
结论
结果强烈表明，使用分流袋对于减少采血过程中的细菌污染具有重要意义。
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