Ozdemir Fethi Ahmet, Yildirim Mehmet Ugur, Pourali Kahriz Mahsa
Department of Molecular Biology and Genetics, Faculty of Sciences, Bartin University, 74100 Bartin, Turkey.
Department of Field Crops, Faculty of Agriculture, Ankara University, Diskapı, 06110 Ankara, Turkey.
Biomed Res Int. 2014;2014:476346. doi: 10.1155/2014/476346. Epub 2014 Aug 27.
Lallemantia iberica (Bieb.) Fisch. and C. A. Mey is high valued annual ornamental and medicinal plant from Lamiaceae family that prefers dry sunny hillsides, roadsides, slopes, and fallow fields over an altitude of 500-2150 m. It bears beautiful white flowers and bloom from April to June each year. This study reports L. iberica micropropagation using cotyledon node explants isolated from 15-day-old in vitro regenerated plantlets. The cotyledon node explants were cultured on MS medium containing 0.50, 1.00 plus 2.00 mg/L BAP, 0.00, 0.01, and 0.02 mg/L NAA. Maximum shoot regeneration was noted on MS medium containing 0.50 mg/L BAP. Well-developed micropropagated shoots were rooted on MS medium containing 1.00 mg/L IBA. The rooted plants were easily hardened in the growth chamber and acclimatised in greenhouse.
伊朗琉璃苣(Lallemantia iberica (Bieb.) Fisch. and C. A. Mey)是唇形科一年生高价值观赏和药用植物,喜生于海拔500 - 2150米的干燥向阳山坡、路边、斜坡和休耕地。它开美丽的白色花朵,每年4月至6月开花。本研究报道了利用从15日龄离体再生幼苗分离的子叶节外植体进行伊朗琉璃苣的微繁殖。子叶节外植体接种在添加了0.50、1.00和2.00毫克/升苄氨基嘌呤(BAP)以及0.00、0.01和0.02毫克/升萘乙酸(NAA)的MS培养基上。在添加0.50毫克/升BAP的MS培养基上观察到最高的芽再生率。发育良好的微繁殖芽在添加1.00毫克/升吲哚丁酸(IBA)的MS培养基上生根。生根植株在生长室中易于炼苗,并在温室中驯化。