Zebarjadi Alireza, Kazem Samaneh, Kahrizi Danial
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran.
Cell Mol Biol (Noisy-le-grand). 2018 May 30;64(7):92-96.
Arctium lappa L. (Burdock) is an important plant with various pharmacological effects. According to the importance of this plant, optimization of its tissue culture will lead to more investigation and application of it. The aim of this study was to develop protocols for callus induction and shoot regeneration of A. lappa. In order to optimize of tissue culture in A. lappa, callus induction, indirect regeneration and direct regeneration were carried out in factorial experiment based on Completely Randomized Designs (CRDs). Hypocotyl and cotyledon were cultured on the Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 6-Benzylaminopurine (BAP) for callus induction. In indirect regeneration experiment various levels of BAP and α-Naphthaleneacetic acid (NAA) and two types of explants (calli derived from cotyledon and hypocotyl) were investigated. In direct regeneration section, various levels of BAP plus 2 mg/l NAA and different explants (cotyledon, hypocotyl and bud) were compared. In both cotyledon and hypocotyl, the maximum callus induction was observed on a media containing 2 mg/l 2,4-D plus 1 mg/l BAP (100% and 76.19% respectively). The highest percentage of indirect regeneration (65%) was observed at 1 mg/l BAP plus 0.5 mg/l NAA on calli from hypocotyl. The highest percentage of direct regeneration (90.33) was observed in hypocotyl with a lateral bud explant on MS medium supplemented with 0.5 mg/l BAP plus 2 mg/l NAA. In this study, optimization of tissue culture protocol for A. lappa was carried out as a research technique, as well as technique for further exploitation of this plant.
牛蒡是一种具有多种药理作用的重要植物。鉴于这种植物的重要性，优化其组织培养将有助于对其进行更多的研究和应用。本研究的目的是建立牛蒡愈伤组织诱导和芽再生的方案。为了优化牛蒡的组织培养，基于完全随机设计（CRD）进行了析因实验，包括愈伤组织诱导、间接再生和直接再生。将下胚轴和子叶接种在添加了不同浓度和组合的2,4-二氯苯氧乙酸（2,4-D）和6-苄基腺嘌呤（BAP）的Murashige和Skoog（MS）培养基上进行愈伤组织诱导。在间接再生实验中，研究了不同水平的BAP和α-萘乙酸（NAA）以及两种外植体（来自子叶和下胚轴的愈伤组织）。在直接再生部分，比较了不同水平的BAP加2 mg/l NAA和不同外植体（子叶、下胚轴和芽）。在子叶和下胚轴中，在含有2 mg/l 2,4-D加1 mg/l BAP的培养基上观察到最大的愈伤组织诱导率（分别为100%和76.19%）。在下胚轴愈伤组织上，在1 mg/l BAP加0.5 mg/l NAA时观察到最高的间接再生率（65%）。在添加了0.5 mg/l BAP加2 mg/l NAA的MS培养基上，下胚轴带侧芽外植体的直接再生率最高（90.33%）。在本研究中，牛蒡组织培养方案的优化既是一种研究技术，也是进一步开发这种植物的技术。
