Kucharska A M, Gorska E, Stelmaszczyk-Emmel A, Demkow U, Pyrżak B
Department of Pediatrics and Endocrinology, Warsaw Medical University, 24 Marszalkowska St., 00-756, Warsaw, Poland,
Adv Exp Med Biol. 2015;833:47-53. doi: 10.1007/5584_2014_35.
The main cause of autoimmune thyroiditis of Hashimoto's type (HT) is a pathological immune response to thyroid antigens. The aim of the study was to present clinical characteristics and immune profile of children with HT. Ninety five children were examined: 45 with HT (age: 8-18 years) and 50 healthy age-matched controls. The peripheral blood mononuclear cells' (PBMC) phenotype was evaluated using a Beckman Coulter flow cytometer with the following monoclonal antibodies: CD4-FITC, CD28-PC5, CD152-PE and CD8-FITC, CD28-PC5, CD152-PE. The thyroid stimulating hormone, thyroid hormones, and antibodies against thyroid peroxidase (TPO) and thyroglobulin (TG) were evaluated by a microparticle enzyme immunoassay. We found that goiter was present in 53% of children, the thyroid had an increased density in palpation in 98%, and hypothyroidism was diagnosed in 11% of HT patients. The number of CD152+ was lower in HT than in healthy children (p<0.05). CD4+ and CD8+ PBMC subsets did not differ between the groups at baseline. After stimulation with phytohemagglutinine (PHA), CD4+ cells decreased in healthy controls and remained constant in HT children. Anti-TPO and anti-TG antibodies were higher in children with a lower percentage of CD152+. No other markers correlated with the immunological profile of PBMC. The percentages of CD4+ and CD152+ negatively correlated with the anti-TG concentration. We conclude that children with HT have a different PBMC profile than healthy children and show a different pattern of response to stimulation.
桥本氏型自身免疫性甲状腺炎（HT）的主要病因是针对甲状腺抗原的病理性免疫反应。本研究的目的是呈现HT患儿的临床特征和免疫谱。对95名儿童进行了检查：45名HT患儿（年龄8 - 18岁）和50名年龄匹配的健康对照。使用配备以下单克隆抗体的贝克曼库尔特流式细胞仪评估外周血单个核细胞（PBMC）的表型：CD4 - FITC、CD28 - PC5、CD152 - PE以及CD8 - FITC、CD28 - PC5、CD152 - PE。通过微粒体酶免疫测定法评估促甲状腺激素、甲状腺激素以及抗甲状腺过氧化物酶（TPO）和甲状腺球蛋白（TG）的抗体。我们发现53%的患儿存在甲状腺肿大，98%的患儿甲状腺触诊密度增加，11%的HT患者被诊断为甲状腺功能减退。HT患儿中CD152 +的数量低于健康儿童（p < 0.05）。基线时两组之间CD4 +和CD8 + PBMC亚群无差异。用植物血凝素（PHA）刺激后，健康对照组中CD4 +细胞减少，而HT患儿中保持不变。CD152 +百分比较低的儿童中抗TPO和抗TG抗体较高。没有其他标志物与PBMC的免疫谱相关。CD4 +和CD152 +的百分比与抗TG浓度呈负相关。我们得出结论，HT患儿的PBMC谱与健康儿童不同，并且对刺激表现出不同的反应模式。
