Key Laboratory of Biology and Genetic Resources of Tropical Crops , Ministry of Agriculture , Institute of Tropical Bioscience and Biotechnology , Chinese Academy of Tropical Agricultural Sciences , Haikou , China .
Genet Mol Biol. 2014 Sep;37(3):549-55. doi: 10.1590/s1415-47572014000400011.
The cDNA encoding the R1-MYB transcription factor, designated as JcR1MYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcR1MYB1 contains a 951 bp open reading frame that encodes 316 amino acids. The deduced JcR1MYB1 protein was predicted to possess the conserved, 56-amino acid-long DNA-binding domain, which consists of a single helix-turn-helix module and usually occurs in R1-MYBs. JcR1MYB1 is a member of the R1-MYB transcription factor subfamily. A subcellular localization study confirmed the nuclear localization of JcR1MYB1. Expression analysis showed that JcR1MYB1 transcripts accumulated in various examined tissues, with high expression levels in the root and low levels in the stem. JcR1MYB1 transcription was up-regulated by polyethylene glycol, NaCl, and cold treatments, as well as by abscisic acid, jasmonic acid, and ethylene treatment. Analysis of transgenic tobacco plants over-expressing JcR1MYB1 indicates an inportant function for this gene in salt stress.
采用快速扩增 cDNA 末端的方法，从麻疯树中分离出编码 R1-MYB 转录因子的 cDNA，命名为 JcR1MYB1。JcR1MYB1 含有 951 bp 的开放阅读框，编码 316 个氨基酸。推测推测的 JcR1MYB1 蛋白具有保守的、长 56 个氨基酸的 DNA 结合域，由一个单螺旋-转角-螺旋模块组成，通常存在于 R1-MYB 中。JcR1MYB1 是 R1-MYB 转录因子亚家族的成员。亚细胞定位研究证实了 JcR1MYB1 的核定位。表达分析表明，JcR1MYB1 转录本在各种检测组织中积累，在根中表达水平较高，在茎中表达水平较低。JcR1MYB1 的转录受到聚乙二醇、NaCl 和冷处理以及脱落酸、茉莉酸和乙烯处理的上调。过表达 JcR1MYB1 的转基因烟草植物分析表明，该基因在盐胁迫中具有重要功能。
