Zhu Lu, Guan Yunxiao, Liu Yanan, Zhang Zhaohe, Jaffar Muhammad Abuzar, Song Aiping, Chen Sumei, Jiang Jiafu, Chen Fadi
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Hortic Res. 2020 Jul 1;7:96. doi: 10.1038/s41438-020-0317-1. eCollection 2020.
The switch from vegetative growth to reproductive growth is a key event in the development of a plant. Here, the product of the chrysanthemum gene , an R2R3 MYB transcription factor that is localized in the nucleus, was shown to be a component of the switching mechanism. Plants engineered to overexpress flowered earlier than did wild-type plants, while those in which was suppressed flowered later. In both the overexpression and RNAi knockdown plants, a number of genes encoding proteins involved in gibberellin synthesis or signaling, as well as in the response to photoperiod, were transcribed at a level that differed from that in the wild type. Both yeast two-hybrid and bimolecular fluorescence complementation assays revealed that CmMYB2 interacts with CmBBX24, a zinc-finger transcription factor known to regulate flowering by its influence on gibberellin synthesis.
从营养生长向生殖生长的转变是植物发育过程中的一个关键事件。在这里，菊花基因的产物是一种定位于细胞核的R2R3 MYB转录因子，它被证明是这种转变机制的一个组成部分。经过基因工程改造过表达的植株比野生型植株开花更早，而该基因被抑制的植株则开花较晚。在过表达植株和RNA干扰敲除植株中，一些编码参与赤霉素合成或信号传导以及光周期响应相关蛋白质的基因，其转录水平与野生型植株不同。酵母双杂交和双分子荧光互补试验均表明，CmMYB2与CmBBX24相互作用，CmBBX24是一种已知通过影响赤霉素合成来调控开花的锌指转录因子。
