Izadi-Mood Narges, Sarmadi Soheila, Rostamnasl Behzad
Dept. of Pathology, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Cancer Prev. 2013 Fall;6(4):209-13.
Endometrial cancer is the most common gynecologic malignancy that has often proceeded by a premalignant phase. Modern molecular and immunostaining methods for precancerous lesions diagnosis have been expanded. One of the genetic alternations in the endometrial cancer carcinogenesis is the mutational activation of the K-ras oncogene. K-ras mutation has recognized to occur at an early stage of neoplastic progression in the endometrium. The purpose of this study is to investigate the expression pattern of K-ras gene in atypical and nonatypical hyperplastic endometrium.
In a prospective study in the referral gynecologic hospital in Tehran, immunohistochemical evaluation of K-ras has performed on 72 consecutive specimens in two following groups: endometrial hyperplasia without atypia (n: 36), and endometrial hyperplasia with atypia (n: 36). Staining of cells has evaluated in arbitrary quantitative methods in regards to both slides area staining and intensity of color reaction.
K-ras immunoreactivity has seen in 3/36 (8.3%) cases of non-atypical hyperplasia and in 2/36 (5.6%) cases of atypical hyperplasia (P: 0.64).
We have not establish any significant differences in K-ras expression between the atypical and nonatypical hyperplastic endometrium, and our data has supported this view that K-ras mutation is a very rare event in human endometrial carcinogenesis.
子宫内膜癌是最常见的妇科恶性肿瘤，通常有一个癌前阶段。用于癌前病变诊断的现代分子和免疫染色方法已经得到扩展。子宫内膜癌发生过程中的基因改变之一是K-ras癌基因的突变激活。K-ras突变被认为发生在子宫内膜肿瘤进展的早期阶段。本研究的目的是调查K-ras基因在非典型和典型增生性子宫内膜中的表达模式。
在德黑兰一家转诊妇科医院进行的一项前瞻性研究中，对两组共72个连续标本进行了K-ras的免疫组织化学评估：非典型子宫内膜增生（n = 36）和典型子宫内膜增生（n = 36）。就玻片区域染色和颜色反应强度而言，采用任意定量方法评估细胞染色情况。
在3/36（8.3%）例非典型增生病例和2/36（5.6%）例典型增生病例中观察到K-ras免疫反应性（P = 0.64）。
我们未发现非典型和典型增生性子宫内膜之间K-ras表达有任何显著差异，并且我们的数据支持了这一观点，即K-ras突变在人类子宫内膜癌发生过程中是非常罕见的事件。
