Department of Pathology, Mirza Koochak Khan Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Diagn Pathol. 2009 Nov 25;4:41. doi: 10.1186/1746-1596-4-41.
Different molecular alterations have been described in endometrioid endometrial carcinoma (EECA). Among them the most frequently altered is loss of the PTEN protein, a tumor suppressor gene. The purpose of this study was to evaluate the expression pattern of PTEN gene in normal, hyperplastic and neoplastic endometrium.
In a study in a referral gynecologic hospital in Tehran, Iran, immunohistochemical (IHC) evaluation of PTEN was performed on 87 consecutive specimens to the following three groups; group A- normal proliferative endometrium(n = 29); group B- hyperplastic endometrium [including simple hyperplasia without atypia(n = 21) and complex hyperplasia with atypia (n = 8)] and group C- EECA(n = 29). Immunostaining of cells was analyzed by arbitrary quantitative methods according to both slide's area staining and intensity of color reaction.
PTEN immunoreactivity was present in all normal proliferative endometrium, all simple hyperplasia, 75% of atypical complex hyperplasia and in 48% of EECA (P < 0.001). The intensity of PTEN reaction was significantly higher in group with proliferative endometrium than hyperplastic endometrium and EECA (P < 0.001).
PTEN expression was significantly higher in cyclical endometrium than in atypical hyperplasia and endometrioid carcinoma.
子宫内膜样腺癌（EECA）存在不同的分子改变。其中最常见的改变是抑癌基因 PTEN 蛋白的缺失。本研究旨在评估 PTEN 基因在正常、增生和肿瘤性子宫内膜中的表达模式。
在伊朗德黑兰一家转诊妇科医院进行的一项研究中，对 87 例连续标本进行了免疫组织化学（IHC）评估，将其分为三组：A 组-正常增生性子宫内膜（n=29）；B 组-增生性子宫内膜[包括单纯非典型增生（n=21）和复杂非典型增生（n=8）]；C 组-EECA（n=29）。根据玻片的区域染色和颜色反应强度，采用任意定量方法分析细胞免疫染色。
PTEN 免疫反应性存在于所有正常增生性子宫内膜、所有单纯性增生、75%的非典型复杂性增生和 48%的 EECA 中（P<0.001）。在增殖性子宫内膜组中，PTEN 反应的强度明显高于增生性子宫内膜组和 EECA 组（P<0.001）。
PTEN 表达在周期性子宫内膜中明显高于非典型增生和子宫内膜样癌。
