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二氢嘧啶酶相关蛋白（DRPs）表达变化对恶性星形细胞瘤脑肿瘤影响的研究

The Study of "Dihydropyrimidinase Related Proteins (DRPs)" Expression Changes Influence in Malignant Astrocytoma Brain Tumor.

作者信息

Pooladi Mehdi, Rezaei-Tavirani Mostafa, Hashemi Mehrdad, Hesami-Tackallou Saeed, Khaghani-Razi-Abad Solmaz, Moradi Afshin, Zali Ali Reza, Mousavi Masoumea, Rakhshan Azadeh, Firozi-Dalvand Leila, Omidi Roghaye

机构信息

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Dept. of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Cancer Prev. 2014 Summer;7(3):130-6.

PMID:25250163
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4171831/
Abstract

BACKGROUND

Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding.

METHODS

The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks.

RESULTS

The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values.

CONCLUSION

This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity.

摘要

背景

二氢嘧啶酶相关蛋白（DRPs）已知与塌陷反应介导蛋白（CRMPs）同源。DRP基因家族由四个成员组成，即DRP 1、2、3和4，所有这些成员都被认为参与轴突生长和路径寻找。

方法

从肿瘤组织、正常脑组织中提取蛋白质，然后通过Bradford试验和分光光度法评估蛋白质纯度。在本研究中，采用二维凝胶（2DG）电泳法分离蛋白质，然后使用统计数据和特定软件（Progenesis Same Spots）分析和比较斑点。通过pH等电点、分子量和数据库鉴定斑点。

结果

二维凝胶共显示800个斑点。在人类脑蛋白质组中，DRP2有两个斑点被报道，DRP3有一个斑点被报道，它们在pH等电点和分子量值上有所不同。

结论

该蛋白家族参与神经元分化和轴突导向，在发育中的大脑中受到大量影响，但其表达持续到成年期。DRP2受磷酸化调节，糖原合酶激酶3调节DRP2的磷酸化使其处于无活性状态，并诱导神经元极性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/ba549d35e637/IJCP-07-130f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/bb3e6355e71f/IJCP-07-130f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/487c72492e7b/IJCP-07-130f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/a1ea7b033b50/IJCP-07-130f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/1d315a254265/IJCP-07-130f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/ba549d35e637/IJCP-07-130f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/bb3e6355e71f/IJCP-07-130f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/487c72492e7b/IJCP-07-130f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/a1ea7b033b50/IJCP-07-130f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/1d315a254265/IJCP-07-130f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/26b1/4171831/ba549d35e637/IJCP-07-130f5.jpg

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