Yuan Kefei, Liu Yuan, Chen Hai-Ning, Zhang Lu, Lan Jiang, Gao Wei, Dou Qianhui, Nice Edouard C, Huang Canhua
The State Key Laboratory for Biotherapy, West China Hospital, Sichuan University, Chengdu, P. R. China.
Proteomics. 2015 Jan;15(2-3):287-99. doi: 10.1002/pmic.201400164. Epub 2014 Dec 15.
Cancer cells maintain their intracellular ROS concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins (redox sensors). Upon oxidative stress, redox sensors undergo redox modifications that cause the allosteric changes of these proteins and endow them with different functions. Understanding the altered functions of redox sensors and the underlying mechanisms is critical for the development of novel cancer therapeutics. Recently, a series of high-throughput proteomics approaches have been developed for screening redox processes. In this manuscript, we review these methodologies and discuss the important redox sensors recently identified that are related to cancer.
癌细胞将其细胞内活性氧（ROS）浓度维持在生存所需的水平。ROS浓度的变化可调节控制细胞功能的生化信号传导机制。已经证明，ROS通过对氧化还原敏感蛋白（氧化还原传感器）的氧化还原调节来调控细胞事件。在氧化应激时，氧化还原传感器会发生氧化还原修饰，导致这些蛋白质的变构变化并赋予它们不同的功能。了解氧化还原传感器功能的改变及其潜在机制对于开发新型癌症治疗方法至关重要。最近，已经开发出一系列高通量蛋白质组学方法用于筛选氧化还原过程。在本手稿中，我们综述了这些方法，并讨论了最近鉴定出的与癌症相关的重要氧化还原传感器。
