Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, 70803, USA.
J Integr Plant Biol. 2015 Mar;57(3):271-83. doi: 10.1111/jipb.12286. Epub 2014 Nov 17.
Plant β-1,3-glucanases are members of the pathogenesis-related protein 2 (PR-2) family, which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses. One of the differentially expressed proteins (spot 842) identified in a recent proteomic comparison between five pairs of closely related maize (Zea mays L.) lines differing in aflatoxin resistance was further investigated in the present study. Here, the corresponding cDNA was cloned from maize and designated as ZmGns. ZmGns encodes a protein of 338 amino acids containing a potential signal peptide. The expression of ZmGns was detectible in all tissues studied with the highest level in silks. ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus. ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid. In vivo, ZmGns showed a significant inhibitory activity against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis. Its high level of expression in the silk tissue and its induced expression by phytohormone treatment, as well as by bacterial and fungal infections, suggest it plays a complex role in maize growth, development, and defense.
植物β-1,3-葡聚糖酶是病程相关蛋白 2（PR-2）家族的成员之一，该家族是 17 个 PR 蛋白家族之一，在生物和非生物胁迫反应中发挥重要作用。在最近对五对密切相关的玉米（Zea mays L.）品系进行的蛋白质组比较中，发现了一种差异表达蛋白（斑点 842），本研究对其进行了进一步研究。在这里，从玉米中克隆出相应的 cDNA，并将其命名为 ZmGns。ZmGns 编码一个含有潜在信号肽的 338 个氨基酸的蛋白质。ZmGns 的表达在所有研究的组织中均可检测到，在丝中表达水平最高。ZmGns 被三种细菌和真菌黄曲霉诱导表达。ZmGns 还被大多数测试的非生物胁迫和生长激素如水杨酸诱导表达。在体内，ZmGns 在拟南芥中过表达时对细菌病原体丁香假单胞菌 pv.番茄 DC3000 和真菌病原体 Botrytis cinerea 表现出显著的抑制活性。其在丝组织中的高水平表达及其对植物激素处理、细菌和真菌感染的诱导表达表明，它在玉米的生长、发育和防御中发挥着复杂的作用。
