Chen Dian-Yang, Chen Qiu-Yi, Wang Dan-Dan, Mu Yu-Pei, Wang Mu-Yang, Huang Ji-Rong, Mao Ying-Bo
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.
CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, University of CAS, Chinese Academy of Sciences, Shanghai, China.
Front Plant Sci. 2020 Sep 15;11:573131. doi: 10.3389/fpls.2020.573131. eCollection 2020.
The green mirid bug () and the cotton bollworm () are both preferred to live on cotton but cause different symptoms, suggesting specialized responses of cotton to the two insects. In this study, we investigated differential molecular mechanisms underlying cotton plant defenses against and transcriptomic analyses. At the transcription level, jasmonate (JA) signaling was dominated in defense against whereas salicylic acid (SA) signaling was more significant in defense against . A set of pathogenesis-related (PR) genes and protease inhibitor genes were differentially induced by the two insects. Insect infestations also had an impact on alternative splicing (AS), which was altered more significantly by the than . Interestingly, most differential AS (DAS) genes had no obvious change at the transcription level. GO analysis revealed that biological process termed "RNA splicing" and "cellular response to abiotic stimulus" were enriched only in DAS genes from the infested samples. Furthermore, insect infestations induced the retained intron of GhJAZs transcripts, which produced a truncated protein lacking the intact Jas motif. Taken together, our data demonstrate that the specialized cotton response to different insects is regulated by gene transcription and AS as well.
绿盲蝽（）和棉铃虫（）都偏好寄生于棉花上，但会引发不同症状，这表明棉花对这两种昆虫有特异性反应。在本研究中，我们通过转录组分析探究了棉花植株抵御和的不同分子机制。在转录水平上，茉莉酸（JA）信号通路在抵御时占主导地位，而水杨酸（SA）信号通路在抵御时更为显著。一组病程相关（PR）基因和蛋白酶抑制剂基因被这两种昆虫差异诱导。昆虫侵害也对可变剪接（AS）产生影响，相比，的影响更为显著。有趣的是，大多数差异可变剪接（DAS）基因在转录水平上没有明显变化。基因本体（GO）分析表明，“RNA剪接”和“细胞对非生物刺激反应”这两个生物学过程仅在受侵害样本的DAS基因中富集。此外，昆虫侵害诱导了GhJAZs转录本的内含子保留，产生了一种缺少完整Jas基序的截短蛋白。综上所述，我们的数据表明棉花对不同昆虫的特异性反应也受基因转录和可变剪接的调控。
