Li P, Ma H, Han D, Mou K
Center for Translational Medicine, College of Medicine, First Affiliated Hospital Xi'an Jiaotong University, Xi'an, China.
Clin Exp Dermatol. 2015 Mar;40(2):163-70. doi: 10.1111/ced.12464. Epub 2014 Sep 23.
Vitiligo is an acquired skin pigmentation disorder. Interleukin (IL)-33 is a newly described member of the IL-1 family.
To examine the role of IL-33 and its regulation in vitiligo.
Expression of IL-33 and its receptor (interleukin 1 receptor-like 1 protein; also known as ST2), in skin biopsies taken from healthy subjects and patients with vitiligo, was examined by immunofluorescence staining and western blotting. IL-33 secretion from primary keratinocytes was measured by ELISA. IL-33-stimulated release of stem cell factor (SCF), basic fibroblast growth factor (bFGF), IL-6 and tumour necrosis factor (TNF)-α from primary keratinocytes was examined by ELISA.
IL-33 and ST2 expression was increased in lesional skin, and serum IL-33 was raised in patients with vitiligo. IL-33 expression moved from the nucleus to the cytoplasm of keratinocytes. IL-33 reduced expression of both SCF and bFGF, but increased expression of both IL-6 and TNF-α expression in primary keratinocytes.
IL-33 is secreted by keratinocytes and functions as an alarmin. It may induce melanocyte death by regulating cytokines in the cellular microenvironment.
白癜风是一种获得性皮肤色素沉着障碍。白细胞介素（IL）-33是IL-1家族新发现的成员。
研究IL-33在白癜风中的作用及其调控机制。
采用免疫荧光染色和蛋白质印迹法检测健康受试者和白癜风患者皮肤活检组织中IL-33及其受体（白细胞介素1受体样1蛋白，也称为ST2）的表达。采用酶联免疫吸附测定（ELISA）法检测原代角质形成细胞分泌IL-33的水平。采用ELISA法检测IL-33刺激原代角质形成细胞释放干细胞因子（SCF）、碱性成纤维细胞生长因子（bFGF）、IL-6和肿瘤坏死因子（TNF）-α的水平。
白癜风患者皮损处皮肤中IL-33和ST2表达增加，血清IL-33水平升高。IL-33在角质形成细胞中的表达从细胞核转移至细胞质。IL-33可降低原代角质形成细胞中SCF和bFGF的表达，但可增加IL-6和TNF-α的表达。
角质形成细胞可分泌IL-33，其作为警报素发挥作用。它可能通过调节细胞微环境中的细胞因子诱导黑素细胞死亡。
