Hall Brad, Jones Lyndon, Forrest James A
Centre for Contact Lens Research, School of Optometry and Vision Science , Waterloo, Ontario , Canada and.
Curr Eye Res. 2015 May;40(6):622-31. doi: 10.3109/02713683.2014.946518. Epub 2014 Sep 24.
To determine the effect of competitive adsorption between lysozyme and lactoferrin on silicone hydrogel contact lenses and the effect on lysozyme activity.
Three commercially available silicone hydrogel contact lens materials (senofilcon A, lotrafilcon B and balafilcon A) were examined, for time points ranging from 10 s to 2 h. Total protein deposition was determined by I(125) radiolabeling of lysozyme and lactoferrin, while the activity of lysozyme was determined by a micrococcal activity assay.
Senofilcon A and balafilcon A did not show any relevant competitive adsorption between lysozyme and lactoferrin. Lotrafilcon B showed reduced protein deposition due to competitive adsorption for lactoferrin at all time points and lysozyme after 7.5 min. Co-adsorption of lactoferrin and lysozyme decreased the activity of lysozyme in solution for senofilcon A and lotrafilcon B, but co-adsorption had no effect on the surface activity of lysozyme for all lens types investigated.
Competition between lysozyme and lactoferrin is material specific. Co-adsorption of lysozyme and lactoferrin does not affect the activity of surface-bound lysozyme but can reduce the activity of subsequently desorbed lysozyme.
确定溶菌酶和乳铁蛋白之间的竞争性吸附对硅水凝胶隐形眼镜的影响以及对溶菌酶活性的影响。
检测了三种市售的硅水凝胶隐形眼镜材料（senofilcon A、lotrafilcon B和balafilcon A），时间点从10秒至2小时。通过对溶菌酶和乳铁蛋白进行I(125)放射性标记来测定总蛋白沉积，同时通过微球菌活性测定法来测定溶菌酶的活性。
Senofilcon A和balafilcon A在溶菌酶和乳铁蛋白之间未显示出任何相关的竞争性吸附。Lotrafilcon B在所有时间点均显示出由于对乳铁蛋白的竞争性吸附导致的蛋白沉积减少，在7.5分钟后对溶菌酶也有竞争性吸附。对于senofilcon A和lotrafilcon B，乳铁蛋白和溶菌酶的共吸附降低了溶液中溶菌酶的活性，但对于所有研究的镜片类型，共吸附对溶菌酶的表面活性没有影响。
溶菌酶和乳铁蛋白之间的竞争具有材料特异性。溶菌酶和乳铁蛋白的共吸附不会影响表面结合的溶菌酶的活性，但会降低随后解吸的溶菌酶的活性。
