十二指肠空肠旁路术可减轻西方饮食诱导肥胖大鼠的非酒精性脂肪性肝病。

Duodenal jejunal bypass attenuates non-alcoholic fatty liver disease in western diet-obese rats.

作者信息

Ebertz Claudia Emanuelli, Bonfleur Maria Lúcia, Bertasso Iala Milene, Mendes Mariana Carla, Lubaczeuski Camila, Araujo Allan Cezar de Freitas, Paes Antônio Marcus, de Amorim Elaine Manoela Porto, Balbo Sandra Lucinei

机构信息

State University of Western Parana, Cascavel, PR, Brasil.

Center for Biological Sciences and Health, UNIOESTE, Cascavel, PR, Brasil.

出版信息

Acta Cir Bras. 2014 Sep;29(9):609-14. doi: 10.1590/s0102-8650201400150009.

DOI:10.1590/s0102-8650201400150009

PMID:25252208

Abstract

PURPOSE

To evaluate the effects of duodenal-jejunal bypass (DJB) on serum and hepatic profiles of obese rats fed on a western diet (WD).

METHODS

Twenty eight male Wistar rats were fed a standard rodent chow diet (CTL group) or WD ad libitum. After 10 weeks, WD rats were submitted to sham (WD SHAM) or duodenal-jejunal bypass (WD DJB). Body weight, fat pad depots, glycemia, insulinemia, HOMA-IR, TyG, lipids profile and hepatic analyses were evaluated two months after surgery.

RESULTS

The WD SHAM group presented greater obesity, hyperglycemia, hyperinsulinemia, insulin resistance, hypertriglyceridemia and hepatic steatosis than the CTL group. WD DJB rats presented decreased serum glucose and insulin resistance, when compared to WD SHAM animals, without changes in insulinemia. In addition, DJB surgery normalized serum TG and attenuated TG accumulation and steatosis in the liver of the WD DJB group. Hepatic ACC and FAS protein expressions were similar in all groups.

CONCLUSION

Duodenal-jejunal bypass attenuates hepatic parameters of non-alcoholic fatty liver disease in obese rats fed on a western diet.

摘要

目的

评估十二指肠-空肠旁路术（DJB）对喂食西式饮食（WD）的肥胖大鼠血清和肝脏指标的影响。

方法

28只雄性Wistar大鼠随意喂食标准啮齿动物饲料（CTL组）或WD。10周后，WD大鼠接受假手术（WD SHAM）或十二指肠-空肠旁路术（WD DJB）。术后两个月评估体重、脂肪垫储存、血糖、胰岛素血症、HOMA-IR、TyG、血脂谱和肝脏分析。

结果

与CTL组相比，WD SHAM组表现出更严重的肥胖、高血糖、高胰岛素血症、胰岛素抵抗、高甘油三酯血症和肝脂肪变性。与WD SHAM动物相比，WD DJB大鼠的血糖和胰岛素抵抗降低，胰岛素血症无变化。此外，DJB手术使WD DJB组大鼠的血清TG正常化，并减轻了肝脏中TG的积累和脂肪变性。所有组的肝脏ACC和FAS蛋白表达相似。