Zhang Yuting, Wang Minhua, Chen Jiadong, Rong Junkang, Ding Mingguan
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an 311300, China; The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Yi Chuan. 2014 Sep;36(9):921-33. doi: 10.3724/SP.J.1005.2014.0921.
Heat shock 70 (HSP70) proteins are highly conserved molecular chaperones widely existed in the plant kingdom which are involved in cellular protein folding process. In this study, comprehensive evolutionary analyses of the Gossypium raimondii HSP70 gene family members are conducted and 30 HSP70 genes are identified. The gene structure, chromosome distribution, gene duplication and phylogenic evolution of this family are further analyzed. The results reveal that HSP70 family genes can be clustered into several major subgroups based on their sub-cellular locations, and the gene structures are relatively conserved in each subgroup. Both tandem duplications and chromosome segmental duplications are found to contribute to the expansion of HSP70 gene family. Evolutionary analysis of HSP70s in diverse species reveals that the differentiation of HSP70 subgroups occurred before the multi-cell and single-cell differentiation, with the cytoplasmic HSP70s multiple amplified. The expression pattern of HSP70 genes under series of fiber development stages indicates that many HSP70 genes may participate in fiber development processes including fiber initiation and elongation. This study provides the complete profiles of cotton HSP70 family genes for future study on their functions related to the molecular mechanisms of fiber development.
热休克70（HSP70）蛋白是广泛存在于植物界的高度保守的分子伴侣，参与细胞内蛋白质折叠过程。本研究对雷蒙德氏棉HSP70基因家族成员进行了全面的进化分析，鉴定出30个HSP70基因。进一步分析了该家族的基因结构、染色体分布、基因复制和系统发育进化。结果表明，HSP70家族基因可根据其亚细胞定位聚类为几个主要亚组，且每个亚组的基因结构相对保守。串联重复和染色体片段重复均对HSP70基因家族的扩张有贡献。对不同物种中HSP70的进化分析表明，HSP70亚组的分化发生在多细胞和单细胞分化之前，细胞质HSP70多次扩增。HSP70基因在一系列纤维发育阶段的表达模式表明，许多HSP70基因可能参与纤维发育过程，包括纤维起始和伸长。本研究为今后研究棉花HSP70家族基因与纤维发育分子机制相关的功能提供了完整的概况。
