Childress H, Sullivan B, Kaur J, Karthikeyan R
Department of Biological and Agricultural Engineering, Texas A&M University, 2117 TAMU, College Station, TX 77843-2117, USA E-mail:
J Water Health. 2014 Sep;12(3):404-9. doi: 10.2166/wh.2013.257.
The ubiquitous use of antibiotics has led to an increasing number of antibiotic-resistant bacterial strains, including strains that are multidrug-resistant, pathogenic, or both. There is also evidence to suggest that antibiotic resistance genes (ARGs) spread to the environment, humans, and animals through wastewater effluents. The overall objective of this study was to investigate the effect of ultraviolet (UV) light disinfection on antibiotic-resistant bacteria. Wastewater effluent samples from a wastewater treatment plant (WWTP) in Texas were evaluated for differences in tetracycline-resistant bacteria before and after UV treatment. The effects of photoreactivation or dark repair on the reactivation of bacteria present in WWTP effluent after UV disinfection were also examined. Culture-based methods were used to characterize viable heterotrophic, tetracycline-resistant heterotrophic, Escherichia coli, and tetracycline-resistant E. coli bacteria present before and after UV treatment. UV disinfection was found to be as effective at reducing concentrations of resistant heterotrophs and E. coli, as it was at reducing total bacterial concentrations. The lowest survival ratio following UV disinfection was observed in tetracycline-resistant E. coli showing particular susceptibility to UV treatment. Photoreactivation and dark repair rates were found to be comparable to each other for all bacterial populations.
抗生素的广泛使用导致了越来越多的抗生素耐药菌株的出现，包括多重耐药、致病或兼具这两种特性的菌株。也有证据表明，抗生素抗性基因（ARGs）通过废水排放扩散到环境、人类和动物中。本研究的总体目标是调查紫外线（UV）消毒对耐药细菌的影响。对得克萨斯州一家污水处理厂（WWTP）的废水排放样本进行了评估，以了解紫外线处理前后耐四环素细菌的差异。还研究了光复活或暗修复对紫外线消毒后污水处理厂废水中存在的细菌再活化的影响。采用基于培养的方法对紫外线处理前后存在的活的异养菌、耐四环素异养菌、大肠杆菌和耐四环素大肠杆菌进行表征。发现紫外线消毒在降低耐药异养菌和大肠杆菌浓度方面与降低总细菌浓度一样有效。在耐四环素大肠杆菌中观察到紫外线消毒后的最低存活率，表明其对紫外线处理特别敏感。发现所有细菌群体的光复活率和暗修复率彼此相当。
