State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China.
State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, China.
Chemosphere. 2019 Jun;224:827-832. doi: 10.1016/j.chemosphere.2019.03.004. Epub 2019 Mar 2.
Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are the emerging contaminants leading to a serious worldwide health problem. Although disinfection like ultraviolet (UV) irradiation could remove part of ARB and ARGs, there still are residual ARB and ARGs in the effluent of wastewater treatment plants. Conjugative transfer is main concern of the risk of ARGs and little is known about the effects of UV disinfection on the transfer ability of the non-inactivated ARB in the effluent which will enter the environment. Hence the influences of UV irradiation and reactivation on ARB conjugative transfer ability were studied under laboratory condition, focusing on the survival bacteria from UV irradiation and the reactivated bacteria, as well as their descendants. The experimental results imply that even 1 mJ/cm UV disinfection can significantly decrease the conjugative transfer frequency of the survival bacteria. However, viable but not culturable state cells induced by UV can reactivate through both photoreactivation and dark repair and retain the same level of transfer ability as the untreated strains. This finding is essential for re-considering about the post safety of UV irradiated effluent and microbial safety control strategies were required.
耐药细菌（ARB）和抗生素耐药基因（ARGs）是新兴的污染物，导致了严重的全球健康问题。尽管消毒（如紫外线照射）可以去除部分 ARB 和 ARGs，但在污水处理厂的废水中仍有残留的 ARB 和 ARGs。共轭转移是 ARGs 风险的主要关注点，而对于紫外线消毒对进入环境的非失活 ARB 的转移能力的影响知之甚少。因此，在实验室条件下研究了紫外线照射和再激活对 ARB 共轭转移能力的影响，重点关注了紫外线照射后的存活细菌和再激活细菌及其后代。实验结果表明，即使是 1mJ/cm 的紫外线消毒也可以显著降低存活细菌的共轭转移频率。然而，紫外线诱导的活但不可培养状态细胞可以通过光复活和暗修复重新激活，并保持与未经处理的菌株相同的转移能力。这一发现对于重新考虑紫外线照射后的废水的安全性以及需要微生物安全控制策略至关重要。
