Bellnier D A, Dougherty T J
Photochem Photobiol. 1989 Mar;49(3):369-72. doi: 10.1111/j.1751-1097.1989.tb04121.x.
This study was designed to investigate the time course of acute cutaneous photosensitivity following administration of Photofrin II using the murine ear swelling response (ESR) as an in vivo end-point. Ros:(ICR) mice were injected with 5 mg/kg Photofrin II and illuminated 7.5 h to 31 days later with 630-nm laser light; ESR was measured 24 h after illumination. There was a direct correlation between ESR and the concentration of [14C]Photofrin II in blood, while no relationship between ESR and the level of [14C]Photofrin II in the ear tissue of exsanguinated mice was evident. Photosensitivity in the mouse foot can be suppressed by preexposure to low doses of light via a photochemical destruction of tissue-bound sensitizer (Boyle and Potter, 1987, Photochem. Photobiol. 46, 997-1001). However, mouse ears pretreated with 84 J/cm2 of 630-nm light (28 J/cm2/day, given 2, 4 and 6 d after injection), a dose sufficient to reduce porphyrin fluorescence in ear tissue by about 75%, prior to the usual light dose (88.6 J/cm2, 630 nm, day 9 after injection) showed a mean ESR not significantly different (P less than 0.5) from that for ears which received only a single dose of 88.6 J/cm2 on day 9. It is concluded, for this animal model, that circulating porphyrin is the source of photoinduced ear-tissue edema and that photobleaching of tissue-bound sensitizer does not attenuate ear-tissue photosensitivity.
本研究旨在以小鼠耳部肿胀反应（ESR）作为体内终点，研究注射二血卟啉醚（Photofrin II）后急性皮肤光敏感性的时间进程。将（ICR）小鼠注射5 mg/kg的二血卟啉醚（Photofrin II），并在7.5小时至31天后用630纳米激光照射；照射后24小时测量ESR。ESR与血液中[14C]二血卟啉醚（Photofrin II）的浓度呈直接相关，而在放血小鼠的耳部组织中，ESR与[14C]二血卟啉醚（Photofrin II）的水平之间没有明显关系。通过光化学破坏组织结合的敏化剂，预先暴露于低剂量的光可以抑制小鼠足部的光敏感性（Boyle和Potter，1987年，《光化学与光生物学》46，997 - 1001）。然而，在通常的光照剂量（注射后第9天，88.6 J/cm2，630纳米）之前，用84 J/cm2的630纳米光（28 J/cm2/天，在注射后第2、4和6天给予）预处理小鼠耳部，该剂量足以使耳部组织中的卟啉荧光降低约75%，其平均ESR与仅在第9天接受单剂量88.6 J/cm2照射的耳部相比，差异不显著（P小于0.5）。对于该动物模型，得出的结论是，循环卟啉是光诱导耳部组织水肿的来源，并且组织结合敏化剂的光漂白不会减弱耳部组织的光敏感性。
