Roberts W G, Liaw L H, Berns M W
Department of Developmental Beckman Laser Institute and Medical Clinic, Irvine, CA 92715.
Lasers Surg Med. 1989;9(2):102-8. doi: 10.1002/lsm.1900090204.
Primary sites of subcellular destruction with photofrin II (PfII) and mono-L-aspartyl chlorin e6 (MACE) were determined by transmission electron microscopy (TEM). Potorous tridactylus (PTK2) cells were grown in Rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. Cells were irradiated with laser light and fixed and processed for electron microscopy at various times post-irradiation. The results indicate that PfII initially destroys mitochondria, whereas MACE destroys lysosomes. Both conclusions are consistent with fluorescence subcellular localization studies.
通过透射电子显微镜（TEM）确定了二血卟啉醚（PfII）和单-L-天冬氨酸二氢卟吩e6（MACE）造成亚细胞破坏的主要部位。将长吻袋貂（PTK2）细胞培养在罗斯小室中，并用足以导致100%死亡率的敏化剂浓度孵育24小时。用激光照射细胞，并在照射后的不同时间固定并进行电子显微镜处理。结果表明，PfII最初破坏线粒体，而MACE破坏溶酶体。这两个结论均与亚细胞荧光定位研究一致。
