Ogihara Kikumi, Onda Ken, Sato Reiichiro, Naya Yuko, Ochiai Hideharu
Laboratory of Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
J Vet Med Sci. 2015 Jan;77(1):85-8. doi: 10.1292/jvms.14-0014. Epub 2014 Sep 23.
L-type amino acid transporter 1 (LAT1), the first isotype of amino acid transport system L, transports aromatic and branched amino acids pivotal for fundamental cellular activities such cellular growth and proliferation. LAT1 expression was high only in the brain in contrast to its limited distribution and low level of expression in normal tissues. We found potent LAT1 expression in canine caput epididymis by quantitative RT-PCR and Western blotting analysis. Immnuno-histochemical examination revealed observable LAT1 in microvillous epithelial cells.
L型氨基酸转运体1（LAT1）是氨基酸转运系统L的首个亚型，负责转运对细胞生长和增殖等基本细胞活动至关重要的芳香族和支链氨基酸。与LAT1在正常组织中分布有限且表达水平较低形成对比的是，它仅在大脑中高表达。通过定量逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析，我们发现犬附睾头部有较强的LAT1表达。免疫组织化学检查显示，在微绒毛上皮细胞中可观察到LAT1。
