Ohkame H, Masuda H, Ishii Y, Kanai Y
Third Department of Surgery, Nihon University School of Medicine, Tokyo, Japan.
J Surg Oncol. 2001 Dec;78(4):265-71; discussion 271-2. doi: 10.1002/jso.1165.
It has been said that amino acid transporters play an important role in supplying nutrition to cells and for cell proliferation. In this study, we examined whether LAT1 and 4F2hc are closely related to tumor growth.
Rat colon cancer cells (RCN-9) were injected into the spleen of 12 male rats (inbred F344/DuCrj). In each rat, liver samples including tumor lesions were immunostained with anti-LAT1 and anti-4F2hc antibodies. The staining area of LAT1 and 4F2hc tumor lesions was calculated by computer analysis.
Sixty-eight tumor nodules were observed in 12 livers. Out of the 68 tumor nodules, 36 nodules (52.9%) indicated a positive staining of LAT1 and 32 (47.1%) had a negative staining of LAT1. However, the LAT1 expression was scarcely detected in non-tumor areas. In terms of the 4F2hc expression, there were 56 nodules (82.4%) with 4F2hc positive and 12 (17.6) with 4F2hc negative. In addition, the expression of 4F2hc in non-tumor areas was almost the same as the expression of 4F2hc in tumor lesions. The average tumor size of the group with LAT1 positive and 4F2hc positive (n = 31) was 0.845 +/- 0.232 mm(2), which was significantly larger than that of the group with LAT1 negative and 4F2hc negative group (n = 7) (0.090 +/- 0.028 mm(2)) or the group with LAT1 positive and 4F2hc negative (n = 5) (0.097 +/- 0.025 mm(2)), respectively (P = 0.0017, P = 0.007).
LAT1 was related to tumor growth. We think that LAT1 can possibly enhance its ability to promote tumor growth in cooperation with 4F2hc.
据说氨基酸转运蛋白在为细胞提供营养以及细胞增殖过程中发挥着重要作用。在本研究中，我们检测了LAT1和4F2hc是否与肿瘤生长密切相关。
将大鼠结肠癌细胞（RCN - 9）注入12只雄性大鼠（近交系F344/DuCrj）的脾脏。对每只大鼠的包括肿瘤病灶的肝脏样本用抗LAT1和抗4F2hc抗体进行免疫染色。通过计算机分析计算LAT1和4F2hc肿瘤病灶的染色面积。
在12个肝脏中观察到68个肿瘤结节。在这68个肿瘤结节中，36个结节（52.9%）LAT1染色呈阳性，32个（47.1%）LAT1染色呈阴性。然而，在非肿瘤区域几乎检测不到LAT1表达。就4F2hc表达而言，56个结节（82.4%）4F2hc呈阳性，12个（17.6%）4F2hc呈阴性。此外，非肿瘤区域4F2hc的表达与肿瘤病灶中4F2hc的表达几乎相同。LAT1阳性且4F2hc阳性组（n = 31）的平均肿瘤大小为0.845±0.232平方毫米，显著大于LAT1阴性且4F2hc阴性组（n = 7）（0.090±0.028平方毫米）或LAT1阳性且4F2hc阴性组（n = 5）（0.097±0.025平方毫米），分别为（P = 0.0017，P = 0.007）。
LAT1与肿瘤生长相关。我们认为LAT1可能与4F2hc协同增强其促进肿瘤生长的能力。
