Akimoto K, Hayakawa K, Koga Y, Miyata A, Kangawa K, Matsuo H
Kokyu To Junkan. 1989 Mar;37(3):307-11.
Contents and molecular forms of human atrial natriuretic peptide (hANP) in right and left auricle were analyzed by reverse phase high liquid chromatography (RP-HPLC), coupled with radioimmunoassay for hANP. Analyses were done with auricles taken from 4 autopsied cases without heart disease, and 13 patients with heart disease. Both right and left auricular hANP contents in patients with heart disease were higher than those obtained at autopsy. In patients with mitral stenosis (MS) or mitral regurgitation (MR) who have left atrial pressure and/or volume overload, hANP contents in left auricle were higher than those in right auricle. In addition, three types of molecular forms of hANP, (gamma) type, (alpha, beta, gamma) type, (beta, gamma) type, were observed in both right and left atrium. In patients with MS or MR, (beta, gamma) type or (alpha, beta, gamma) type which have beta-hANP immunoreactivity were observed in 8 out of 9 in patients in left auricle, however, in 4 out of 9 patients in right auricle. Our results suggested that the difference of contents and molecular forms of hANP may reflect the pathophysiological role in heart diseases.
采用反相高效液相色谱法（RP-HPLC）结合人心房钠尿肽（hANP）放射免疫分析法，对右心耳和左心耳中hANP的含量及分子形式进行了分析。分析对象为取自4例无心脏病尸检病例及13例心脏病患者的心耳。心脏病患者右心耳和左心耳的hANP含量均高于尸检病例。在有左心房压力和/或容量超负荷的二尖瓣狭窄（MS）或二尖瓣反流（MR）患者中，左心耳的hANP含量高于右心耳。此外，在右心房和左心房中均观察到三种hANP分子形式，即γ型、（α、β、γ）型、（β、γ）型。在MS或MR患者中，9例左心耳患者中有8例观察到具有β-hANP免疫反应性的（β、γ）型或（α、β、γ）型，而右心耳9例患者中有4例观察到。我们的结果表明，hANP含量和分子形式的差异可能反映了其在心脏病中的病理生理作用。
